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Literature summary for 3.1.21.10 extracted from

  • Canas, C.; Carrasco, B.; Ayora, S.; Alonso, J.C.
    The RecU Holliday junction resolvase acts at early stages of homologous recombination (2008), Nucleic Acids Res., 36, 5242-5249.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)[pLysS] cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
K56A mutant with a putative separation-of-function phenotype, the mutant is about 5times less active than the wild type enzyme in cleavage of Holliday junctions, fails to inhibit the dATPase activity of RecA because it does not bind ssDNA Bacillus subtilis
R71A mutant with a putative separation-of-function phenotype, fails to inhibit the dATPase activity of RecA because it does not bind ssDNA Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P39792
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Holliday junction in DNA + H2O
-
 Bacillus subtilis ?
-
 ?
additional information RecU interacts with RecA and inhibits its single-stranded DNA-dependent dATP hydrolysis Bacillus subtilis ?
-
 ?

Synonyms

Synonyms Comment Organism
Holliday junction resolvase
-
 Bacillus subtilis
RecU
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 Bacillus subtilis