Cloned (Comment) | Organism |
---|---|
carboxy-terminal His-tagged GEN1 is expressed in Escherichia coli | Homo sapiens |
expressed in Saccharomyces cerevisiae strain W303 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
carboxy-terminal His-tagged GEN1 is purified using HisTrap column chromatography, heparin column chromatography, ssDNA column chromatography, and MonoS column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Holliday junction in DNA + H2O | GEN1 resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated, GEN1 leaves ligatable nicks after symmetrical cleavage | Homo sapiens | ? | - |
? | |
Holliday junction in DNA + H2O | Yen1 resolves Holliday junctions by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated, Yen1 leaves ligatable nicks after symmetrical cleavage | Saccharomyces cerevisiae | ? | - |
? | |
Holliday junction X0 + H2O | GEN1 cuts the immobile junction X0 at a unique site located one nucleotide to the 3' side of the junction point | Homo sapiens | ? | - |
? | |
Holliday junction X0 + H2O | Yen1 cuts the immobile junction X0 at a unique site located one nucleotide to the 3' side of the junction point | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GEN1 | - |
Homo sapiens |
Holliday junction resolvase | - |
Homo sapiens |
Holliday junction resolvase | - |
Saccharomyces cerevisiae |
Yen1 | - |
Saccharomyces cerevisiae |