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Literature summary for 3.1.21.10 extracted from
- Cleavage mechanism of human Mus81-Eme1 acting on Holliday-junction structures (2008), Proc. Natl. Acad. Sci. USA, 105, 3757-3762.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| amino acids 260-551 for Mus81 and amino acids 244-571 for Eme1 are cloned for expression in Escherichia coli, the complex is named EcME | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D307A | D307 is critical for catalysis | Homo sapiens |
| D338A/D339A | mutant, lacking potential catalytic residues | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
determined by gel filtration using a 3 microM EcME solution, heterodimer | Homo sapiens |
| 140000 | - |
determined by gel filtration using a 88 microM EcME solution, heterotetramer | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| EcME is purified to apparent homogeneity through affinity chromatography, ion exchange, and gel filtration steps | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | X12, nX12, 3'flap - EcME is able to convert all three substrates to linear duplex product | Homo sapiens | ? | - |
? |  |
| nX12 junction in single-stranded DNA + H2O | - |
Homo sapiens | ? | - |
? | |
| X12 junction in single-stranded DNA + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | one molecule of EcMus81 and one molecule of EcEme1 | Homo sapiens |
| tetramer | - |
Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mus81-Eme1 endonuclease | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
endonuclease assay on pAT25 | Homo sapiens |
| 37 | - |
ligation assay | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0047 | - |
X12 junction in single-stranded DNA | like nX12, without the nick at the cross-over point | Homo sapiens | |
| 0.12 | - |
3'-flapped junction in DNA | the 3'flap substrate contains 50 bp of duplex DNA, a central nick, and 25 bases of single-stranded DNA | Homo sapiens | |
| 0.18 | - |
nX12 junction in single-stranded DNA | the nX12 substrate has four 25-bp duplex DNA arms, with a 12-bp homologous core that allows branch migration of the junction, and contains a nick at the cross-over point | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
endonuclease assay on pAT25 | Homo sapiens |
| 8 | - |
ligation assay | Homo sapiens |
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Release 2023.1 (January 2023)
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