Any feedback?
Literature summary for 3.1.21.10 extracted from
- Saccharomyces cerevisiae Mus81-Mms4 is a catalytic, DNA structure-selective endonuclease (2008), Nucleic Acids Res., 36, 2182-2195.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of MUS81 and MMS4 into GAL1/10 divergent promotor, 2 micro-based overexpression vector, based on pJN58 | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D414A/D415A | mutations introduce a diagnostic NheI restriction site | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000012 | - |
D-loop junction in DNA | - |
Saccharomyces cerevisiae | |
| 0.0000031 | - |
nXO12 junction in single-stranded DNA | - |
Saccharomyces cerevisiae | |
| 0.0000055 | - |
3'-flapped junction in DNA | - |
Saccharomyces cerevisiae | |
| 0.0000056 | - |
pXO12-3' junction in single-stranded DNA | - |
Saccharomyces cerevisiae | |
| 0.0000073 | - |
replication fork-like junction in DNA | - |
Saccharomyces cerevisiae | |
| 0.0000304 | - |
splayed Y junction in DNA | - |
Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Saccharomyces cerevisiae |  |
| Mn2+ | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73600 | - |
predicted molecular weight for His10-FLAG-Mus81, verified by PAGE | Saccharomyces cerevisiae |
| 106400 | - |
predicted molecular weight for GST-Mms4, verified by PAGE | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | - |
Saccharomyces cerevisiae |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overexpressed GST-Mms4/His10-Flag-Mus81 is purified by sequential affinity chromatography from Saccharomyces cerevisiae cells | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3'-flapped junction in DNA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| D-loop junction in DNA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | class I substrates reflect low Km and high kcat and include the nicked Holliday junction, 3'-flapped and replication fork-like structures | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | class II substrates share low Km but low kcat relative to class I substrates and include the D-loop and partial Holliday junction | Saccharomyces cerevisiae | ? | - |
? | |
| additional information | class III substrates are defined by splayed Y junctions having high Km and low Kcat | Saccharomyces cerevisiae | ? | - |
? | |
| nXO12 junction in single-stranded DNA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| pXO12-3' junction in single-stranded DNA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| replication fork-like junction in DNA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
| splayed Y junction in DNA + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | - |
Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mus81-Mms4 endonuclease | - |
Saccharomyces cerevisiae |
| Mus81-Mms4/Eme1 endonuclease | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
nuclease assay | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
D-loop junction in DNA | - |
Saccharomyces cerevisiae | |
| 0.26 | - |
splayed Y junction in DNA | - |
Saccharomyces cerevisiae | |
| 0.32 | - |
pXO12-3' junction in single-stranded DNA | - |
Saccharomyces cerevisiae | |
| 0.97 | - |
3'-flapped junction in DNA | - |
Saccharomyces cerevisiae | |
| 1.2 | - |
nXO12 junction in single-stranded DNA | - |
Saccharomyces cerevisiae | |
| 1.35 | - |
replication fork-like junction in DNA | - |
Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
nuclease assay | Saccharomyces cerevisiae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
