Literature summary for 3.1.21.10 extracted from

Culyba, M.J.; Minkah, N.; Hwang, Y.; Benhamou, O.M.; Bushman, F.D.
DNA branch nuclease activity of vaccinia A22 resolvase (2007), J. Biol. Chem., 282, 34644-34652.

Search for more literature for 3.1.21.10

Application

Application	Comment	Organism
medicine	development of antiviral agents against poxviruses	Vaccinia virus

Cloned(Commentary)

Cloned (Comment)	Organism
cloned into the pDuetMxe vector for overexpression in Escherichia coli BL21DE3pLysS cells	Vaccinia virus

Protein Variants

Protein Variants	Comment	Organism
D151A	mutant with amino acid active site substitution	Vaccinia virus
D151N	mutant with amino acid active site substitution	Vaccinia virus
D152A	mutant with amino acid active site substitution	Vaccinia virus
D152N	mutant with amino acid active site substitution	Vaccinia virus
D155A	mutant with amino acid active site substitution	Vaccinia virus
D30A	mutant with amino acid active site substitution	Vaccinia virus
D30N	mutant with amino acid active site substitution	Vaccinia virus
E81A	mutant with amino acid active site substitution	Vaccinia virus
E81Q	mutant with amino acid active site substitution	Vaccinia virus
K124A	mutant with amino acid active site substitution	Vaccinia virus
R121A	mutant with amino acid active site substitution	Vaccinia virus

Organism

Organism	UniProt	Comment	Textmining
Vaccinia virus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purified by dual affinity chromatography	Vaccinia virus

Synonyms

Synonyms	Comment	Organism
A22 resolvase	-	Vaccinia virus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	resolvase cleavage assay	Vaccinia virus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	resolvase cleavage assay	Vaccinia virus

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Release 2023.1 (January 2023)