Literature summary for 3.1.21.10 extracted from

McGregor, N.; Ayora, S.; Sedelnikova, S.; Carrasco, B.; Alonso, J.C.; Thaw, P.; Rafferty, J.
The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage (2005), Structure, 13, 1341-1351.

Search for more literature for 3.1.21.10

Cloned(Commentary)

Cloned (Comment)	Organism
in Escherichia coli strain BL21(DE3)(pLysS) containing the recU overexpression plasmid pCB210	Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment)	Organism
at 17°C, hanging drop method, in solution having a mushroom-like appearance, where the cap is formed by the main beta-sheet and flanking helices, structure of RecU superimposes best with PfHjc and archaeal HJR, the next best matches to RecU are a series of type II restriction endonucleases including EcoRV and Pvull	Bacillus subtilis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	Asp88 and Glu101 essential for cation binding	Bacillus subtilis
Mn2+	-	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	P39792	-	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration on a Hi-Load (1.6 x 60 cm) Superdex 200 column, 90% purified by SDS-PAGE	Bacillus subtilis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA + H2O	Holliday structure, RecU acts by making two simultaneous cuts in the phosphodiester backbone via its two active sites	Bacillus subtilis	?	-	?

Subunits

Subunits	Comment	Organism
dimer	determination of structure by MIR	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
RecU endonuclease	-	Bacillus subtilis
RecU Holliday junction resolvase	-	Bacillus subtilis

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Release 2023.1 (January 2023)