Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli using vector pET19b-Hje with a native N-terminal | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000066 | - |
DNA junction 1 | at 15 mM MgCl2, 65°C, pH 7 | Saccharolobus solfataricus | |
0.000073 | - |
DNA junction 1 | at 5 mM MgCl2, 65°C, pH 7 | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | initiation of reaction with 15 mM MgCl2 at 65°C | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified to homogeneity | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA junction 1 + H2O | DNA junction 1 substrate assembled from strands b50, h50,r50,x50, r55, b1-27, b28-50 | Saccharolobus solfataricus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endonuclease Hje | - |
Saccharolobus solfataricus |
Hje | - |
Saccharolobus solfataricus |
Hje endonuclease | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
steady-state approximation at 0.30 min-1 under maximal velocity conditions at 15 mM MgCl2 | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 65 | no multiple turnover catalysis at lower temperatures measurable due to the sensitivity of the assay | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
DNA junction 1 | at 30°C, steady-state approximation under maximal velocity conditions at 15 mM MgCl2 | Saccharolobus solfataricus | |
0.05 | - |
DNA junction 1 | at 65°C, pH 7, converting 6% of the substrate into product | Saccharolobus solfataricus |