Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli containing pMALc2 | Lambdavirus lambda |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | minor junction cleavage | Lambdavirus lambda | |
Mg2+ | required for chi DNA cleavage, 0.1 mM is sufficient to promote stacking of the junction arms, at 1 mM no Rap-junction complexes were detected | Lambdavirus lambda | |
Mn2+ | required for chi DNA cleavage, reduced preference for cleavage in Mn2+ relative to Mg2+ on chi compared to small DNA substrates | Lambdavirus lambda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lambdavirus lambda | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose and heparin agarose chromatography of MBP-Rap, Rap29K, Rap-S and Rap17K | Lambdavirus lambda |
Storage Stability | Organism |
---|---|
-80°C, Tris-HCl buffer, 50% glycerol | Lambdavirus lambda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Rap mediates symmetrical resolution of 50bp and chi Holliday structures containing larger homologous cores | Lambdavirus lambda | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RAP | - |
Lambdavirus lambda |