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Literature summary for 3.1.21.10 extracted from

  • Garcia, A.D.; Otero, J.; Lebowitz, J.; Schuck, P.; Moss, B.
    Quaternary structure and cleavage specificity of a poxvirus holliday junction resolvase (2006), J. Biol. Chem., 281, 11618-11626.
    View publication on PubMed
Search for more literature for 3.1.21.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of MBP-RuvC in Escherichia coli strain ER2508 Escherichia coli
expression of MBP-RuvC in Escherichia coli strain ER2508 Vaccinia virus

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
no activity in mammalia
-
-
-
Vaccinia virus
-
 pox virus family
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on amylose resin and gel filtration of MBP-A22 Vaccinia virus
gel filtration of MBP-RuvC Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O Holliday structure HJ Jun3 Vaccinia virus ?
-
 ?
DNA + H2O Holliday structure HJ X12 Vaccinia virus ?
-
 ?
DNA + H2O Holliday structure HJ-1 Escherichia coli ?
-
 ?
DNA + H2O Holliday structure HJ-1 Vaccinia virus ?
-
 ?
DNA + H2O Holliday structure HJbm4 Escherichia coli ?
-
 ?
DNA + H2O Holliday structure HJbm4 Vaccinia virus ?
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer, 2 * 121930-135000, sedimentation and multi-angle light scattering Escherichia coli
dimer homodimer, 67000-158000, gel filtration, chemical cross linking, sedimentation and multi-angle light scattering Vaccinia virus

Synonyms

Synonyms Comment Organism
Holliday junction resolvase
-
 Escherichia coli
Holliday junction resolvase
-
 Vaccinia virus
MBP-RuvC
-
 Escherichia coli
MBP-RuvC
-
 Vaccinia virus
RuvC
-
 Escherichia coli