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Literature summary for 3.1.21.10 extracted from
- Biochemical characterization of the hjc holliday junction resolvase of Pyrococcus furiosus (2000), Nucleic Acids Res., 28, 4544-4551.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | optimal concentration in reaction buffer: 200 mM | Pyrococcus furiosus |  |
| MgCl2 | required, optimal concentration: 5-10 mM | Pyrococcus furiosus | |
| MnCl2 | can substitute for MgCl2, less efficient | Pyrococcus furiosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | the enzyme efficiently cleaves four-way junctions and less efficiently three-way junctions. The cleaved strand is rejoined by ligation, which means that cleavage occurs at the symmetrically related sites of the two strands, to leave 5'-phosphate and 3'-hydroxyl termini | Pyrococcus furiosus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hjc holliday junction resolvase | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 75 | - |
Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Pyrococcus furiosus |
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Release 2023.1 (January 2023)
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