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Literature summary for 3.1.21.10 extracted from

  • Bolt, E.L.; Sharples, G.J.; Lloyd, R.G.
    Analysis of conserved basic residues associated with DNA binding (Arg69) and catalysis (Lys76) by the RusA holliday junction resolvase (2000), J. Mol. Biol., 304, 165-176.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
K76Q failure of the mutant enzyme to promote DNA repair Escherichia coli
K76R failure of the mutant enzyme to promote DNA repair Escherichia coli
N73A mutant enzyme with 20% of the wild-type activity at 50 nM protein concentration Escherichia coli
R69A failure of the mutant enzyme to promote DNA repair Escherichia coli
R69Q failure of the mutant enzyme to promote DNA repair Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal concentration: 10 mM Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
the enzyme RusA resolvase is encoded by a cryptic rusA gene of the defective pyrophage DLP12
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O catalytic centre: Asp70, Asp72 and Asp91 Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
RusA
-
Escherichia coli
RusA Holliday junction resolvase the enzyme is encoded by a cryptic rusA gene of the defective pyrophage DLP12 Escherichia coli