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Literature summary for 3.1.21.10 extracted from

  • Wardleworth, B.N.; Kvaratskhelia, M.; White, M.F.
    Site-directed mutagenesis of the yeast resolving enzyme Cce1 reveals catalytic residues and relationship with the intron-splicing factor Mrs1 (2000), J. Biol. Chem., 275, 23725-23728.
    View publication on PubMed
Search for more literature for 3.1.21.10

Protein Variants

Protein Variants Comment Organism
D292N 1.2fold decrease in binding of four-way DNA junction, 80fold decrease in catalytic activity Candida albicans
D293N 1.2fold decrease in binding of four-way DNA junction, more than 200fold decrease in catalytic activity Candida albicans
D294N 1.2fold decrease in binding of four-way DNA junction, more than 200fold decrease in catalytic activity Candida albicans
E145Q 0.85fold decrease in binding of four-way DNA junction, more than 200fold decrease in catalytic activity Candida albicans
E231A 21fold decrease in binding of four-way DNA junction, 4fold decrease in catalytic activity Candida albicans
F79A 70fold decrease in binding of four-way DNA junction, more than 200fold decrease in catalytic activity Candida albicans
K291A 130fold decrease in binding of four-way DNA junction, 47fold decrease in catalytic activity Candida albicans
K291A 30fold decrease in binding of four-way DNA junction, more than 200fold decrease in catalytic activity Candida albicans
K291R 130fold decrease in binding of four-way DNA junction, more than 200fold decrease in catalytic activity Candida albicans
Q147A 7.5fold decrease in binding of four-way DNA junction, 100fold decrease in catalytic activity Candida albicans
R146A 70fold decrease in binding of four-way DNA junction, more than 200fold decrease in catalytic activity Candida albicans
R150A 18fold decrease in binding of four-way DNA junction, 70fold decrease in catalytic activity Candida albicans
R231K 330fold decrease in binding of four-way DNA junction, more than 200fold decrease in catalytic activity Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O 32P-labeled four-way DNA junction J1 or junction Z1 Candida albicans ?
-
 ?

Synonyms

Synonyms Comment Organism
Holliday junction-resolving enzyme Cce1
-
 Candida albicans