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Literature summary for 3.1.21.10 extracted from

  • Ariyoshi, M.; Vassylyev, D.; Iwasaki, H.; Fujishima, A.; Shinagawa, H.; Morikawa, K.
    Preliminary chrystallographic study of Escherichia coli RuvC protein (1994), J. Mol. Biol., 241, 281-282.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
three distinct crystal forms, form I: triclinic space group P1, resolution: 4 A, form II: not grown to a size sufficient for X-ray diffraction, form III: monoclinic space group P2-1, resolution: 2.5 A Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cations required for activation Escherichia coli
Mg2+
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18600
-
calculation from amino acid sequence Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Escherichia coli holliday structure hydrolyzed DNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
highly purified Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O nicks the ssDNA strands across the junction at symmetrical positions within the homologous arms Escherichia coli hydrolyzed DNA
-
?
DNA + H2O cleavage at or near the point of strand exchange Escherichia coli hydrolyzed DNA
-
?
DNA + H2O holliday structure Escherichia coli hydrolyzed DNA
-
?