Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimum: 15-30 mM MgCl2, 10% of maximal activity in 4 mM MgCl2 | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Saccharolobus solfataricus | holliday structure | hydrolyzed DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial purification | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | introduces paired nicks into opposing continous strands of a stacked X-junction, independent of local sequence, cleaves an Y-junction in the presence of a bulge in one strand so that stacking of the junction becomes possible | Saccharolobus solfataricus | hydrolyzed DNA | - |
? | |
DNA + H2O | holliday structure | Saccharolobus solfataricus | hydrolyzed DNA | - |
? | |
Holliday junction 3 in DNA + H2O | - |
Saccharolobus solfataricus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Hje endonuclease | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00014 | - |
Holliday junction 3 in DNA | at 50°C | Saccharolobus solfataricus | |
0.02 | - |
Holliday junction 3 in DNA | prediction: at 80°C | Saccharolobus solfataricus |