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Literature summary for 3.1.21.10 extracted from
- Interaction of the resolving enzyme YDC2 with the four-way DNA junction (1998), Nucleic Acids Res., 26, 5609-5616.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| YDC2 | Schizosaccharomyces pombe |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E226N | inactive mutant of YDC2 | Schizosaccharomyces pombe |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Schizosaccharomyces pombe | holliday structure | hydrolyzed DNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| about 95% pure | Schizosaccharomyces pombe |
| YDC2 | Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | nicks favorably the continous strand at the point of strand exchange | Schizosaccharomyces pombe | hydrolyzed DNA | - |
? | |
| DNA + H2O | binds to the X-junction in two specific complexes I and II, unfolds the stacked X-structure, cleaves at a subset of 5'-CT and 5'-TT sequences | Schizosaccharomyces pombe | hydrolyzed DNA | - |
? | |
| DNA + H2O | holliday structure | Schizosaccharomyces pombe | hydrolyzed DNA | - |
? | |
| DNA junction 1 unconstrained + H2O | - |
Schizosaccharomyces pombe | ? | - |
? | |
| DNA junction J1T1 + H2O | - |
Schizosaccharomyces pombe | ? | - |
? | |
| DNA junction J1T2 + H2O | - |
Schizosaccharomyces pombe | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | up to 2 dimers can bind to an X-junction | Schizosaccharomyces pombe |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000006 | - |
DNA junction 1 unconstrained | E226N, 480fold reduced turnover rate | Schizosaccharomyces pombe | |
| 0.00034 | - |
DNA junction J1T2 | - |
Schizosaccharomyces pombe | |
| 0.001 | - |
DNA junction J1T1 | - |
Schizosaccharomyces pombe | |
| 0.0029 | - |
DNA junction 1 unconstrained | - |
Schizosaccharomyces pombe |
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