Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia coli | holliday structure | hydrolyzed DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
RuvC | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | optimal sequence for cleavage: A equally well as T - TT - cutting site- C better than G or A, fastest when cleavage occurs at point of strand exchange, cleavage still possible 1 nucleotide 3' of this position when directed by the sequence, Y-junctions containing the sequence are cleavable | Escherichia coli | hydrolyzed DNA | - |
? | |
DNA + H2O | holliday structure | Escherichia coli | hydrolyzed DNA | - |
? | |
DNA junction RC1 + H2O | - |
Escherichia coli | ? | - |
? | |
DNA junction RC1(1,3') + H2O | - |
Escherichia coli | ? | - |
? | |
DNA junction RC1(2,3') + H2O | - |
Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000047 | - |
DNA junction RC1(2,3') | - |
Escherichia coli | |
0.000566 | - |
DNA junction RC1(1,3') | - |
Escherichia coli | |
0.00416 | - |
DNA junction RC1 | - |
Escherichia coli |