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Literature summary for 3.1.21.10 extracted from

  • Fogg, J.M.; Schofield, M.J.; White, M.F.; Lilley, D.M.J.
    Sequence and functional-group specificity for cleavage of DNA junctions by RuvC of Escherichia coli (1999), Biochemistry, 38, 11349-11358.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA + H2O Escherichia coli holliday structure hydrolyzed DNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
RuvC Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O optimal sequence for cleavage: A equally well as T - TT - cutting site- C better than G or A, fastest when cleavage occurs at point of strand exchange, cleavage still possible 1 nucleotide 3' of this position when directed by the sequence, Y-junctions containing the sequence are cleavable Escherichia coli hydrolyzed DNA
-
?
DNA + H2O holliday structure Escherichia coli hydrolyzed DNA
-
?
DNA junction RC1 + H2O
-
Escherichia coli ?
-
?
DNA junction RC1(1,3') + H2O
-
Escherichia coli ?
-
?
DNA junction RC1(2,3') + H2O
-
Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
dimer homodimer Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000047
-
DNA junction RC1(2,3')
-
Escherichia coli
0.000566
-
DNA junction RC1(1,3')
-
Escherichia coli
0.00416
-
DNA junction RC1
-
Escherichia coli