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Literature summary for 3.1.21.10 extracted from
- Partial purification of an enzyme from Saccharomyces cerevisiae that cleaves Holliday junctions (1985), Proc. Natl. Acad. Sci. USA, 82, 7247-7251.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | Saccharomyces cerevisiae | holliday structure | hydrolyzed DNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 500-1000fold | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00000583 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.6 ml enzyme solution were diluted with 0.6 ml glycerol | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | specifically cleaves Holliday junctions, e.g. in bacteriophage G4 figure-8 molecules, cleavage at either of two sites present in the stem of the cruciform, not at the end of the stem | Saccharomyces cerevisiae | hydrolyzed DNA | - |
? | |
| DNA + H2O | holliday structure | Saccharomyces cerevisiae | hydrolyzed DNA | - |
? | |
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Release 2023.1 (January 2023)
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