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Literature summary for 3.1.21.1 extracted from

  • Anindya, R.; Savithri, H.S.
    Potyviral NIa proteinase, a proteinase with novel deoxyribonuclease activity (2004), J. Biol. Chem., 279, 32159-32169.
    View publication on PubMed
Search for more literature for 3.1.21.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Potyvirus sp.

Protein Variants

Protein Variants Comment Organism
C151A activity similar to wild type Potyvirus sp.
D81G no activity Potyvirus sp.
D81N 50% activity compared to wild type Potyvirus sp.
H46A activity similar to wild type Potyvirus sp.

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibition observed above 50 microM, complete inhibition at 0.5 mM Potyvirus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2
-
 Potyvirus sp.

Organism

Organism UniProt Comment Textmining
Potyvirus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography and gel filtration, highly pure protein was used for assays Potyvirus sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Potyvirus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded circular DNA + H2O
-
 Potyvirus sp. ?
-
 ?
double-stranded linear DNA + H2O
-
 Potyvirus sp. ?
-
 ?
lambda phage DNA + H2O
-
 Potyvirus sp. ?
-
 ?

Synonyms

Synonyms Comment Organism
Potyviral Ia Proeinase
-
 Potyvirus sp.
PVBV proteinase
-
 Potyvirus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0214
-
 lambda phage DNA 37°C, 20 mM Tris-HCl pH 8.3, 1 mM MgCl2 Potyvirus sp.
0.0214
-
 (25R)-3beta-hydroxycholest-5-en-27-oate 37°C, 20 mM Tris-HCl pH 8.3, 1 mM MgCl2 Potyvirus sp.

pH Range

pH Minimum pH Maximum Comment Organism
8
-
 best activity above pH 8.0 Potyvirus sp.