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Literature summary for 3.1.21.1 extracted from

  • Takeshita, H.; Yasuda, T.; Iida, R.; Nakajima, T.; Mori, S.; Mogi, K.; Kaneko, Y.; Kishi, K.
    Amphibian DNases I are characterized by a C-terminal end with a unique, cysteine-rich stretch and by the insertion of a serine residue into the Ca2+-binding site (2001), Biochem. J., 357, 473-480.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.21.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression in COS-7 cells Bufo japonicus
DNA and amino acid sequence determination and analysis, expression in COS-7 cells Cynops pyrrhogaster
DNA and amino acid sequence determination and analysis, expression in COS-7 cells as His-tagged enzyme Lithobates catesbeianus
DNA and amino acid sequence determination and analysis, expression in COS-7 cells as His-tagged enzyme Cynops pyrrhogaster

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by G-actin Bufo japonicus
additional information no inhibition by G-actin; no inhibition by G-actin Cynops pyrrhogaster
additional information no inhibition by G-actin Lithobates catesbeianus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ essential, binding site characterization Lithobates catesbeianus
Ca2+ essential, binding site characterization Cynops pyrrhogaster
Ca2+ essential, binding site characterization Bufo japonicus
Mg2+
-
 Lithobates catesbeianus
Mg2+
-
 Cynops pyrrhogaster
Mg2+
-
 Bufo japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Lithobates catesbeianus
40000
-
 gel filtration Cynops pyrrhogaster
40000
-
 gel filtration Bufo japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dsDNA + H2O Lithobates catesbeianus
-
 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
dsDNA + H2O Cynops pyrrhogaster
-
 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
dsDNA + H2O Bufo japonicus
-
 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
additional information Lithobates catesbeianus phylogenetic analysis ?
-
 ?
additional information Cynops pyrrhogaster phylogenetic analysis ?
-
 ?
additional information Bufo japonicus phylogenetic analysis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bufo japonicus Q90WM0
-
-
Cynops pyrrhogaster
-
-
-
Cynops pyrrhogaster Q90W31
-
-
Lithobates catesbeianus Q90WM1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Lithobates catesbeianus
no glycoprotein
-
 Cynops pyrrhogaster
no glycoprotein
-
 Bufo japonicus

Purification (Commentary)

Purification (Comment) Organism
1300fold from pancreas Bufo japonicus
1400fold from pancreas Cynops pyrrhogaster
1800fold from pancreas Lithobates catesbeianus
1800fold from pancreas Cynops pyrrhogaster

Source Tissue

Source Tissue Comment Organism Textmining
additional information no activity in cerebrum, heart, lung, liver, kidney, spleen, skin, muscle, oesophagus, stomach, duodenum, and large intestine Lithobates catesbeianus
-
additional information no activity in cerebrum, heart, lung, liver, kidney, spleen, skin, muscle, oesophagus, stomach, duodenum, and large intestine Cynops pyrrhogaster
-
additional information no activity in cerebrum, heart, lung, liver, kidney, spleen, skin, muscle, oesophagus, stomach, duodenum, and large intestine Bufo japonicus
-
pancreas high activity Lithobates catesbeianus
-
pancreas high activity Cynops pyrrhogaster
-
pancreas high activity Bufo japonicus
-
small intestine low activity Lithobates catesbeianus
-
small intestine low activity Cynops pyrrhogaster
-
small intestine low activity Bufo japonicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 purified enzyme Lithobates catesbeianus
additional information
-
 purified enzyme Cynops pyrrhogaster
additional information
-
 purified enzyme Bufo japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + H2O
-
 Lithobates catesbeianus 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
dsDNA + H2O
-
 Cynops pyrrhogaster 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
dsDNA + H2O
-
 Bufo japonicus 5'-phosphodinucleotide + 5'-phosphooligonucleotide
-
 ?
additional information phylogenetic analysis Lithobates catesbeianus ?
-
 ?
additional information phylogenetic analysis Cynops pyrrhogaster ?
-
 ?
additional information phylogenetic analysis Bufo japonicus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Lithobates catesbeianus
monomer 1 * 40000, SDS-PAGE Cynops pyrrhogaster
monomer 1 * 40000, SDS-PAGE Bufo japonicus
More structural analysis, insertion of a serine residue at position 205 and addition of a cysteine-rich C-stretch at the C-terminus are significant Lithobates catesbeianus
More structural analysis, insertion of a serine residue at position 205 and addition of a cysteine-rich C-stretch at the C-terminus are significant Cynops pyrrhogaster
More structural analysis, insertion of a serine residue at position 205 and addition of a cysteine-rich C-stretch at the C-terminus are significant Bufo japonicus

Synonyms

Synonyms Comment Organism
DNase I
-
 Lithobates catesbeianus
DNase I
-
 Cynops pyrrhogaster
More enzyme belongs to the DNase I family Lithobates catesbeianus
More enzyme belongs to the DNase I family Cynops pyrrhogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 bell-shaped optimum Lithobates catesbeianus
8
-
 bell-shaped optimum Cynops pyrrhogaster
8
-
 bell-shaped optimum Bufo japonicus