Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 80% inhibition at 0.1 mM | Homo sapiens | |
EGTA | activity inhibited | Homo sapiens | |
G-actin | complete inhibition at 0.001 mg | Homo sapiens | |
iodoacetate | complete inhibition | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secreted into duodenal juice | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation synergistic with Mg2+ | Homo sapiens | |
Mg2+ | optimal concentration 5 mM | Homo sapiens | |
Mg2+ | activation synergistic with Ca2+ | Homo sapiens | |
Mn2+ | 120% activity of Mg2+ activated enzyme at 10 mM | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
double-stranded DNA + H2O | Homo sapiens | hydrolysis of both double- and single-stranded DNA | 5'-phosphooligonucleotides + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
near homogeneity by fractionation steps | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
duodenal juice | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.033 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 10 mM Tris-HCl, pH 7.5, 5 mM CaCl2, 30% polyethylene glycol, more than 3 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
double-stranded DNA + H2O | hydrolysis of both double- and single-stranded DNA | Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
heat-denatured DNA + H2O | - |
Homo sapiens | 5'-phosphooligonucleotides + ? | - |
? | |
supercoiled plasmid DNA + H2O | - |
Homo sapiens | linear DNA + ? | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
rather stable up to | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
stable for 7 days | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.6 | in 33 mM Tris-HCl buffer | Homo sapiens |