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Literature summary for 3.1.21.1 extracted from
- Purification and properties of a neutral endodeoxyribonuclease from guinea pig epidermis (1981), Biochim. Biophys. Acta, 656, 183-188.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| G-actin | complete inhibition at 0.05 mg/ml | Cavia porcellus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Cavia porcellus |  |
| Mn2+ | - |
Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33000 | - |
sucrose density gradient centrifugation, gel filtration | Cavia porcellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| double-stranded DNA + H2O | Cavia porcellus | - |
5'-phosphooligonucleotides + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Cavia porcellus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| near homogeneity by affinity chromatography | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epidermis | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.05 | - |
- |
Cavia porcellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20 mM Tris-HCl, pH 7.5, 5 mM CaCl2, 10% v/v glycerol, 6 months | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA + H2O | degradation of native DNA is 80%, that for denatured DNA 70% | Cavia porcellus | 5'-phosphooligonucleotides + ? | 88% of products are longer than tetranecleotides | ? | |
| double-stranded DNA + H2O | - |
Cavia porcellus | 5'-phosphooligonucleotides + ? | - |
? | |
| heat-denatured DNA + H2O | - |
Cavia porcellus | 5'-phosphooligonucleotides + ? | - |
? | |
| supercoiled plasmid DNA + H2O | - |
Cavia porcellus | linear DNA + ? | superhelical form converted to circular-relaxed and linear forms | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
in the presence of Co2+ in 50 mM cacodylate-HCl buffer | Cavia porcellus |
| 7.5 | - |
in the presence of Mn2+ in 50 mM Tris-HCl buffer | Cavia porcellus |
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