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Literature summary for 3.1.21.1 extracted from

  • Yasuda, T.; Awazu, S.; Sato, W.; Iida, R.; Tanaka, Y.; Kishi, K.
    Human gentically polymorphic deoxyribonuclease: purification, characterization, and multiplicity of urine deoxyribonuclease I (1990), J. Biochem., 108, 393-398.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Homo sapiens
EGTA inhibition at 1 mM Homo sapiens
G-actin 50% inhibition at 0.002 mg/ml Homo sapiens
NaCl decreasing activity with increasing ionic strength Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted into urine Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Co2+ activation at 10 mM Homo sapiens
Mg2+ activation at 10 mM, 2fold activation, if 1 mM CaCl2 is added Homo sapiens
Mn2+ activation at 10 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
SDS-PAGE, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Homo sapiens
-
5'-phosphooligonucleotides + ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains fucose, mannose, galactose, glucosamine, sialic acid Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by 6step chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
urine
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
units defined as absorbance at 260 nm/ml assay solution Homo sapiens
additional information
-
1683 units/mg protein Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA + H2O degrades native DNA 2times faster than denatured DNA Homo sapiens 5'-phosphooligonucleotides + ?
-
?
double-stranded DNA + H2O
-
Homo sapiens 5'-phosphooligonucleotides + ?
-
?
heat-denatured DNA + H2O
-
Homo sapiens 5'-phosphooligonucleotides + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable up to, more stable up to 60°C, if Ca2+ is present Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
in the presence of 10 mM MgCl2 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5
-
Homo sapiens