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Literature summary for 3.1.21.1 extracted from

  • Nakamura, L.T.; Wisnieski, B.J.
    Characterization of the deoxyribonuclease activity of diphtheria toxin (1990), J. Biol. Chem., 265, 5237-5241.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Corynebacterium diphtheriae
EGTA
-
Corynebacterium diphtheriae
KCl less inhibitory than NaCl Corynebacterium diphtheriae
NaCl inhibitory at concentrations below 100 mM Corynebacterium diphtheriae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secreted into the culture medium Corynebacterium diphtheriae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ in the presence of 3 mM Mg2+, activation at 2 mM Corynebacterium diphtheriae
Mg2+ in the presence of 2 mM Ca2+, activation at 3 mM Corynebacterium diphtheriae
Mn2+ increasing activation up to 30 mM Corynebacterium diphtheriae
Zn2+ peaked activity at 0.1 mM Corynebacterium diphtheriae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Corynebacterium diphtheriae
-
5'-phosphooligonucleotides + ?
-
?

Organism

Organism UniProt Comment Textmining
Corynebacterium diphtheriae
-
phage beta infected
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O
-
Corynebacterium diphtheriae 5'-phosphooligonucleotides + ?
-
?
linearized plasmid-DNA + H2O
-
Corynebacterium diphtheriae 5'-phosphooligonucleotides + H2O
-
?
supercoiled plasmid DNA + H2O
-
Corynebacterium diphtheriae linear DNA + ? formation of a relaxed circle that subsequently converts to linear DNA if it is nicked on the other strand at the same position ?

Synonyms

Synonyms Comment Organism
diphtheria toxin
-
Corynebacterium diphtheriae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Corynebacterium diphtheriae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
no activity Corynebacterium diphtheriae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Corynebacterium diphtheriae

pH Range

pH Minimum pH Maximum Comment Organism
5 9
-
Corynebacterium diphtheriae