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Literature summary for 3.1.21.1 extracted from

  • Dwyer, A.A.; Huang, A.J.; Pan, C.Q.; Lazarus, R.A.
    Expression and characterization of a DNase I-Fc fusion enzyme (1999), J. Biol. Chem., 274, 9738-9743.
    View publication on PubMed
Search for more literature for 3.1.21.1

Application

Application Comment Organism
medicine inhaled DNase I into the airways reduces viscoelasticity of sputum, improves lung function of cystic fibrosis patients Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Sf9 cells as a DNaseI-Fc fusion- and a non-fusion protein Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NaCl 10fold lower activity at 150 mM Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation synergistic with Mg2+ Homo sapiens
Mg2+ activation synergistic with Ca2+ Homo sapiens
Mn2+ employs more of the cutting mechanism of supercoiled plasmid-DNA Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
double-stranded DNA + H2O Homo sapiens predominantly by a single-stranded nicking mechanism in the presence of both Ca2+ and Mg2+ 5'-phosphooligonucleotides + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpressed DNaseI-Fc fusion protein Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.008
-
 as plasmid nicking activities in the presence of both Mg2+ and Ca2+ for a DNaseI-Fc fusion protein Homo sapiens
0.096
-
 as plasmid nicking activities in the presence of both Mg2+ and Ca2+ Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
double-stranded DNA + H2O predominantly by a single-stranded nicking mechanism in the presence of both Ca2+ and Mg2+ Homo sapiens 5'-phosphooligonucleotides + ?
-
 ?
linearized plasmid-DNA + H2O
-
 Homo sapiens 5'-phosphooligonucleotides + H2O
-
 ?
p-nitrophenyl phenylphosphonate + H2O
-
 Homo sapiens p-nitrophenol + phenylphosphoric acid
-
 ?
supercoiled plasmid DNA + H2O
-
 Homo sapiens linear DNA + ? formation of a relaxed circle that subsequently converts to linear DNA if it is nicked on the other strand at the same position ?

Subunits

Subunits Comment Organism
monomer 1 * 34000, SDS-PAGE Homo sapiens