Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 2fold activation | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
structural models based on crystallization data | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
Y185F | 2.5fold increase in Km-value | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione | 50% inhibition at 0.0016 mM; 50% inhibition at 0.02 mM | Plasmodium falciparum | |
S-(N-hydroxy-N-chlorophenylcarbamoyl)glutathione | 50% inhibition at 0.002 mM; 50% inhibition at 0.03 mM | Plasmodium falciparum | |
S-(N-hydroxy-N-phenylcarbamoyl)glutathione | 50% inhibition at 0.006 mM; 50% inhibition at 0.185 mM | Plasmodium falciparum | |
S-(p-azidophenacyl)glutathione | - |
Plasmodium falciparum | |
S-hexylglutathione | - |
Plasmodium falciparum | |
S-propylglutathione | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
S-Lactoylglutathione | 25°C | Plasmodium falciparum | |
0.19 | - |
S-Lactoylglutathione | 37°C | Plasmodium falciparum | |
0.225 | - |
S-Lactoylglutathione | 25°C | Plasmodium falciparum | |
0.463 | - |
S-Lactoylglutathione | 37°C, mutant Y185F | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Plasmodium falciparum | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.26 mol per mol of enzyme | Plasmodium falciparum | |
Zn2+ | 1.7 mol per mol of enzyme | Plasmodium falciparum | |
Zn2+ | 0.05 mol per mol of enzyme | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30500 | - |
x * 30500, calculated | Plasmodium falciparum |
31800 | - |
x * 31800, calculated, N-terminally truncated active form | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q5J7B5 | isoform glyoxalase IIB | - |
Plasmodium falciparum | Q71KM2 | glyoxalase II preceded by a targeting sequence | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-lactoylglutathione + H2O | - |
Plasmodium falciparum | D-lactate + glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30500, calculated | Plasmodium falciparum |
? | x * 31800, calculated, N-terminally truncated active form | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
cGloII | - |
Plasmodium falciparum |
tGloII | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
64.3 | - |
S-Lactoylglutathione | 25°C | Plasmodium falciparum | |
70 | - |
S-Lactoylglutathione | 25°C | Plasmodium falciparum | |
119.6 | - |
S-Lactoylglutathione | 25°C | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.8 | - |
Plasmodium falciparum |
7.6 | 7.8 | - |
Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione | 25°C | Plasmodium falciparum | |
0.0007 | - |
S-(N-hydroxy-N-chlorophenylcarbamoyl)glutathione | 25°C | Plasmodium falciparum | |
0.006 | - |
S-(N-hydroxy-N-phenylcarbamoyl)glutathione | 25°C | Plasmodium falciparum | |
0.03 | - |
S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione | 25°C | Plasmodium falciparum | |
0.05 | - |
S-(N-hydroxy-N-chlorophenylcarbamoyl)glutathione | 25°C | Plasmodium falciparum | |
1.3 | - |
S-(p-azidophenacyl)glutathione | 25°C | Plasmodium falciparum | |
1.5 | - |
S-hexylglutathione | 25°C | Plasmodium falciparum | |
1.9 | - |
S-propylglutathione | 25°C | Plasmodium falciparum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Plasmodium falciparum | calculated, N-terminally truncated active form | - |
7.25 |
Plasmodium falciparum | calculated | - |
7.64 |