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Literature summary for 3.1.2.6 extracted from

  • Akoachere, M.; Iozef, R.; Rahlfs, S.; Deponte, M.; Mannervik, B.; Creighton, D.J.; Schirmer, H.; Becker, K.
    Characterization of the glyoxalases of the malarial parasite Plasmodium falciparum and comparison with their human counterparts (2005), Biol. Chem., 386, 41-52.
    View publication on PubMed
Search for more literature for 3.1.2.6

Activating Compound

Activating Compound Comment Organism Structure
Zn2+ 2fold activation Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
structural models based on crystallization data Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
Y185F 2.5fold increase in Km-value Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione 50% inhibition at 0.0016 mM; 50% inhibition at 0.02 mM Plasmodium falciparum
S-(N-hydroxy-N-chlorophenylcarbamoyl)glutathione 50% inhibition at 0.002 mM; 50% inhibition at 0.03 mM Plasmodium falciparum
S-(N-hydroxy-N-phenylcarbamoyl)glutathione 50% inhibition at 0.006 mM; 50% inhibition at 0.185 mM Plasmodium falciparum
S-(p-azidophenacyl)glutathione
-
 Plasmodium falciparum
S-hexylglutathione
-
 Plasmodium falciparum
S-propylglutathione
-
 Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 S-Lactoylglutathione 25°C Plasmodium falciparum
0.19
-
 S-Lactoylglutathione 37°C Plasmodium falciparum
0.225
-
 S-Lactoylglutathione 25°C Plasmodium falciparum
0.463
-
 S-Lactoylglutathione 37°C, mutant Y185F Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Plasmodium falciparum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.26 mol per mol of enzyme Plasmodium falciparum
Zn2+ 0.05 mol per mol of enzyme Plasmodium falciparum
Zn2+ 1.7 mol per mol of enzyme Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30500
-
 x * 30500, calculated Plasmodium falciparum
31800
-
 x * 31800, calculated, N-terminally truncated active form Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q5J7B5 isoform glyoxalase IIB
-
Plasmodium falciparum Q71KM2 glyoxalase II preceded by a targeting sequence
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-lactoylglutathione + H2O
-
 Plasmodium falciparum D-lactate + glutathione
-
 ?

Subunits

Subunits Comment Organism
? x * 30500, calculated Plasmodium falciparum
? x * 31800, calculated, N-terminally truncated active form Plasmodium falciparum

Synonyms

Synonyms Comment Organism
cGloII
-
 Plasmodium falciparum
tGloII
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
64.3
-
 S-Lactoylglutathione 25°C Plasmodium falciparum
70
-
 S-Lactoylglutathione 25°C Plasmodium falciparum
119.6
-
 S-Lactoylglutathione 25°C Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.8
-
 Plasmodium falciparum
7.6 7.8
-
 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0005
-
 S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione 25°C Plasmodium falciparum
0.0007
-
 S-(N-hydroxy-N-chlorophenylcarbamoyl)glutathione 25°C Plasmodium falciparum
0.006
-
 S-(N-hydroxy-N-phenylcarbamoyl)glutathione 25°C Plasmodium falciparum
0.03
-
 S-(N-hydroxy-N-bromophenylcarbamoyl)glutathione 25°C Plasmodium falciparum
0.05
-
 S-(N-hydroxy-N-chlorophenylcarbamoyl)glutathione 25°C Plasmodium falciparum
1.3
-
 S-(p-azidophenacyl)glutathione 25°C Plasmodium falciparum
1.5
-
 S-hexylglutathione 25°C Plasmodium falciparum
1.9
-
 S-propylglutathione 25°C Plasmodium falciparum

pI Value

Organism Comment pI Value Maximum pI Value
Plasmodium falciparum calculated, N-terminally truncated active form
-
 7.25
Plasmodium falciparum calculated
-
 7.64