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Literature summary for 3.1.2.6 extracted from

  • Zang, T.M.; Hollman, D.A.; Crawford, P.A.; Crowder, M.W.; Makaroff, C.A.
    Arabidopsis glyoxalase II contains a zinc/iron binuclear metal center that is essential for substrate binding and catalysis (2001), J. Biol. Chem., 276, 4788-4795.
    View publication on PubMed

Application

Application Comment Organism
pharmacology enzyme is a potential anti-cancer and/or anti-protozoal target, rational design of inhibitors Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type and mutants in Escherichia coli BL21(DE3) Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C140A site-directed mutagenesis, increased kcat, unaltered Km compared to the wild-type enzyme Arabidopsis thaliana
D58C site-directed mutagenesis, highly reduced kcat, unaltered Km compared to the wild-type enzyme Arabidopsis thaliana
H172R site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme Arabidopsis thaliana
H54N site-directed mutagenesis, highly reduced kcat, 2fold increased Km compared to the wild-type enzyme Arabidopsis thaliana
H59C site-directed mutagenesis, reduced activity compared to the wild-type enzyme Arabidopsis thaliana
K142A site-directed mutagenesis, 50% reduced kcat, 2fold increased Km compared to the wild-type enzyme Arabidopsis thaliana
N178A site-directed mutagenesis, reduced kcat, slightly increased Km compared to the wild-type enzyme Arabidopsis thaliana
R225A site-directed mutagenesis, increased activity compared to the wild-type enzyme Arabidopsis thaliana
R248W site-directed mutagenesis, about 8fold reduced kcat, about 10fold increased Km compared to the wild-type enzyme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Arabidopsis thaliana
0.06
-
S-D-lactoylglutathione mutant R225A, pH 7.2, 25°C Arabidopsis thaliana
0.062
-
S-D-lactoylglutathione mutant C140A, pH 7.2, 25°C Arabidopsis thaliana
0.063
-
S-D-lactoylglutathione wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
0.076
-
S-D-lactoylglutathione mutant D58C, pH 7.2, 25°C Arabidopsis thaliana
0.098
-
S-D-lactoylglutathione mutant N178A, pH 7.2, 25°C Arabidopsis thaliana
0.13
-
S-D-lactoylglutathione mutant H54N, pH 7.2, 25°C Arabidopsis thaliana
0.17
-
S-D-lactoylglutathione mutant K142A, pH 7.2, 25°C Arabidopsis thaliana
0.6
-
S-D-lactoylglutathione mutant R248W, pH 7.2, 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isozyme Arabidopsis thaliana 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ cytosolic isozyme, enzyme contains a zinc/iron binuclear metal center that is essential for substrate binding and catalysis, content of wild-type and mutant enzymes, overview Arabidopsis thaliana
Zn2+ cytosolic isozyme, enzyme contains a zinc/iron binuclear metal center that is essential for substrate binding and catalysis, content of wild-type and mutant enzymes, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana the enzyme participates in the detoxification of cytotoxic and mutagenic 2-oxoaldehydes ?
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O24496 gene GLX2-2, cytosolic isozyme
-

Reaction

Reaction Comment Organism Reaction ID
S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate active site and reaction mechanism Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of wild-type and mutants in crude extracts Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme participates in the detoxification of cytotoxic and mutagenic 2-oxoaldehydes Arabidopsis thaliana ?
-
?
S-D-lactoylglutathione + H2O
-
Arabidopsis thaliana glutathione + D-lactate
-
?

Synonyms

Synonyms Comment Organism
acetoacetylglutathione hydrolase
-
Arabidopsis thaliana
Germ cell specific protein
-
Arabidopsis thaliana
GLO II
-
Arabidopsis thaliana
glyoxalase II
-
Arabidopsis thaliana
hydrolase, acetoacetylglutathione
-
Arabidopsis thaliana
hydrolase, hydroxyacylglutathione
-
Arabidopsis thaliana
Round spermatid protein RSP29
-
Arabidopsis thaliana
S-2-hydroxylacylglutathione hydrolase
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.6
-
S-D-lactoylglutathione mutant D58C, pH 7.2, 25°C Arabidopsis thaliana
10.9
-
S-D-lactoylglutathione mutant H54N, pH 7.2, 25°C Arabidopsis thaliana
484
-
S-D-lactoylglutathione mutant R248W, pH 7.2, 25°C Arabidopsis thaliana
600
-
S-D-lactoylglutathione mutant R225A, pH 7.2, 25°C Arabidopsis thaliana
1190
-
S-D-lactoylglutathione mutant N178A, pH 7.2, 25°C Arabidopsis thaliana
1760
-
S-D-lactoylglutathione mutant K142A, pH 7.2, 25°C Arabidopsis thaliana
3930
-
S-D-lactoylglutathione wild-type enzyme, pH 7.2, 25°C Arabidopsis thaliana
6780
-
S-D-lactoylglutathione mutant C140A, pH 7.2, 25°C Arabidopsis thaliana