Application | Comment | Organism |
---|---|---|
pharmacology | enzyme is a potential anti-cancer and/or anti-protozoal target, rational design of inhibitors | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutants in Escherichia coli BL21(DE3) | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C140A | site-directed mutagenesis, increased kcat, unaltered Km compared to the wild-type enzyme | Arabidopsis thaliana |
D58C | site-directed mutagenesis, highly reduced kcat, unaltered Km compared to the wild-type enzyme | Arabidopsis thaliana |
H172R | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
H54N | site-directed mutagenesis, highly reduced kcat, 2fold increased Km compared to the wild-type enzyme | Arabidopsis thaliana |
H59C | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Arabidopsis thaliana |
K142A | site-directed mutagenesis, 50% reduced kcat, 2fold increased Km compared to the wild-type enzyme | Arabidopsis thaliana |
N178A | site-directed mutagenesis, reduced kcat, slightly increased Km compared to the wild-type enzyme | Arabidopsis thaliana |
R225A | site-directed mutagenesis, increased activity compared to the wild-type enzyme | Arabidopsis thaliana |
R248W | site-directed mutagenesis, about 8fold reduced kcat, about 10fold increased Km compared to the wild-type enzyme | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Arabidopsis thaliana | |
0.06 | - |
S-D-lactoylglutathione | mutant R225A, pH 7.2, 25°C | Arabidopsis thaliana | |
0.062 | - |
S-D-lactoylglutathione | mutant C140A, pH 7.2, 25°C | Arabidopsis thaliana | |
0.063 | - |
S-D-lactoylglutathione | wild-type enzyme, pH 7.2, 25°C | Arabidopsis thaliana | |
0.076 | - |
S-D-lactoylglutathione | mutant D58C, pH 7.2, 25°C | Arabidopsis thaliana | |
0.098 | - |
S-D-lactoylglutathione | mutant N178A, pH 7.2, 25°C | Arabidopsis thaliana | |
0.13 | - |
S-D-lactoylglutathione | mutant H54N, pH 7.2, 25°C | Arabidopsis thaliana | |
0.17 | - |
S-D-lactoylglutathione | mutant K142A, pH 7.2, 25°C | Arabidopsis thaliana | |
0.6 | - |
S-D-lactoylglutathione | mutant R248W, pH 7.2, 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic isozyme | Arabidopsis thaliana | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | cytosolic isozyme, enzyme contains a zinc/iron binuclear metal center that is essential for substrate binding and catalysis, content of wild-type and mutant enzymes, overview | Arabidopsis thaliana | |
Zn2+ | cytosolic isozyme, enzyme contains a zinc/iron binuclear metal center that is essential for substrate binding and catalysis, content of wild-type and mutant enzymes, overview | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | the enzyme participates in the detoxification of cytotoxic and mutagenic 2-oxoaldehydes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O24496 | gene GLX2-2, cytosolic isozyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate | active site and reaction mechanism | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of wild-type and mutants in crude extracts | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme participates in the detoxification of cytotoxic and mutagenic 2-oxoaldehydes | Arabidopsis thaliana | ? | - |
? | |
S-D-lactoylglutathione + H2O | - |
Arabidopsis thaliana | glutathione + D-lactate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetoacetylglutathione hydrolase | - |
Arabidopsis thaliana |
Germ cell specific protein | - |
Arabidopsis thaliana |
GLO II | - |
Arabidopsis thaliana |
glyoxalase II | - |
Arabidopsis thaliana |
hydrolase, acetoacetylglutathione | - |
Arabidopsis thaliana |
hydrolase, hydroxyacylglutathione | - |
Arabidopsis thaliana |
Round spermatid protein RSP29 | - |
Arabidopsis thaliana |
S-2-hydroxylacylglutathione hydrolase | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.6 | - |
S-D-lactoylglutathione | mutant D58C, pH 7.2, 25°C | Arabidopsis thaliana | |
10.9 | - |
S-D-lactoylglutathione | mutant H54N, pH 7.2, 25°C | Arabidopsis thaliana | |
484 | - |
S-D-lactoylglutathione | mutant R248W, pH 7.2, 25°C | Arabidopsis thaliana | |
600 | - |
S-D-lactoylglutathione | mutant R225A, pH 7.2, 25°C | Arabidopsis thaliana | |
1190 | - |
S-D-lactoylglutathione | mutant N178A, pH 7.2, 25°C | Arabidopsis thaliana | |
1760 | - |
S-D-lactoylglutathione | mutant K142A, pH 7.2, 25°C | Arabidopsis thaliana | |
3930 | - |
S-D-lactoylglutathione | wild-type enzyme, pH 7.2, 25°C | Arabidopsis thaliana | |
6780 | - |
S-D-lactoylglutathione | mutant C140A, pH 7.2, 25°C | Arabidopsis thaliana |