Cloned (Comment) | Organism |
---|---|
cytoplasmic gene GLO2 and mitochondrial gene GLO4, expression in Escherichia coli | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31153 | - |
x * 31153, recombinant enzyme from mitochondria, GLO2, MALDI-TOF | Saccharomyces cerevisiae |
31321 | - |
x * 31321, recombinant enzyme from cytosol, GLO2, MALDI-TOF | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-D-mandeloylglutathione + H2O | - |
Saccharomyces cerevisiae | D-mandelate + glutathione | - |
? | |
S-mandeloylglutathione + H2O | - |
Saccharomyces cerevisiae | mandelate + glutathione | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31153, recombinant enzyme from mitochondria, GLO2, MALDI-TOF | Saccharomyces cerevisiae |
? | x * 31321, recombinant enzyme from cytosol, GLO2, MALDI-TOF | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.8 | - |
S-D-mandeloylglutathione | recombinant enzyme from mitochondria | Saccharomyces cerevisiae | |
35.1 | - |
S-D-mandeloylglutathione | recombinant enzyme from cytosol | Saccharomyces cerevisiae | |
38.1 | - |
S-D-mandeloylglutathione | wild-type enzyme from mitochondria | Saccharomyces cerevisiae | |
605 | - |
S-D-lactoylglutathione | wild-type enzyme from mitochondria | Saccharomyces cerevisiae | |
723 | - |
S-D-lactoylglutathione | recombinat enzyme from mitochondria | Saccharomyces cerevisiae | |
979 | - |
S-D-lactoylglutathione | recombinat enzyme from cytosol | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | mitochondrial enzyme | Saccharomyces cerevisiae |
7 | 8 | cytoplasmic enzyme | Saccharomyces cerevisiae |