Any feedback?
Literature summary for 3.1.2.6 extracted from
- Purification and characterization of glyoxalase II from Hansenula mrakii (1992), J. Ferment. Bioeng., 73, 271-276.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Cyberlindnera mrakii |  |
| Hg2+ | HgCl2 | Cyberlindnera mrakii | |
| iodoacetate | - |
Cyberlindnera mrakii | |
| methylglyoxal-glutathione hemimercaptal | - |
Cyberlindnera mrakii | |
| NEM | - |
Cyberlindnera mrakii | |
| PCMB | - |
Cyberlindnera mrakii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.086 | - |
S-Lactoylglutathione | - |
Cyberlindnera mrakii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
gel filtration | Cyberlindnera mrakii |
| 24000 | - |
- |
Cyberlindnera mrakii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera mrakii | - |
IFO 0895 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyberlindnera mrakii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31.9 | - |
- |
Cyberlindnera mrakii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactoylglutathione + H2O | - |
Cyberlindnera mrakii | D-lactate + glutathione | - |
? | |
| S-acetylglutathione + H2O | - |
Cyberlindnera mrakii | acetate + glutathione | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 24000, SDS-PAGE | Cyberlindnera mrakii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
Cyberlindnera mrakii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | 25°C: about 60% of maximal activity, 55°C: about 70% of maximal activity | Cyberlindnera mrakii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
15 min, 15% loss of activity | Cyberlindnera mrakii |
| 55 | - |
15 min, 90% loss of activity | Cyberlindnera mrakii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Cyberlindnera mrakii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | stable | Cyberlindnera mrakii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
