Literature summary for 3.1.2.4 extracted from

Wong, B.J.; Gerlt, J.A.
Divergent function in the crotonase superfamily: an anhydride intermediate in the reaction catalyzed by 3-hydroxyisobutyryl-CoA hydrolase (2003), J. Am. Chem. Soc., 125, 12076-12077.

Search for more literature for 3.1.2.4

Protein Variants

Protein Variants	Comment	Organism
D151N	reduced activity	Pseudomonas aeruginosa
E143A	no detectable activity	Pseudomonas aeruginosa
E143D	no detectable activity	Pseudomonas aeruginosa
E143Q	no detectable activity	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	-	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
3-hydroxy-2-methylpropanoyl-CoA + H2O = CoA + 3-hydroxy-2-methylpropanoate	formation of an anhydride reaction intermediate, catalytic mechanism	Pseudomonas aeruginosa	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-hydroxyisobutyryl-CoA + H2O	formation of an anhydride reaction intermediate, Glu164 is involved	Pseudomonas aeruginosa	CoA + 3-hydroxyisobutyrate	-	?

Synonyms

Synonyms	Comment	Organism
3-hydroxyisobutyryl-CoA hydrolase	-	Pseudomonas aeruginosa
HICH	-	Pseudomonas aeruginosa
More	the enzyme belongs to the crotonase superfamily	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Pseudomonas aeruginosa
1	-	3-hydroxyisobutyryl-CoA	mutant D151N	Pseudomonas aeruginosa
89	-	3-hydroxyisobutyryl-CoA	wild-type enzyme, a deuterium isotope of the substrate is used	Pseudomonas aeruginosa

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)