Protein Variants | Comment | Organism |
---|---|---|
D130A | mutant in KH-like motif, pyocyanin production 0.2 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
D178A | active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
D73A | active site mutant, pyocyanin production 4.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
E182A | active site mutant, pyocyanin production 1.2 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
F195A | active site mutant, pyocyanin production 0.8 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
F276A | active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
H159A | active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
H221A | active site mutant, pyocyanin production 1.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
H282A | C-terminal alpha-helix mutant, pyocyanin production 0.4 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
H69A | active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
H71A | active site mutant, pyocyanin production 2.7 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
H74A | active site mutant, pyocyanin production 3.5 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
K101A | mutant in KH-like motif, pyocyanin production 4.4 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
L193A | active site mutant, pyocyanin production 4.7 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
L248A | C-terminal alpha-helix mutant, pyocyanin production 4.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
L261A | C-terminal alpha-helix mutant, pyocyanin production 0.2 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
L277A | active site mutant, pyocyanin production 4.5 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
L290A | C-terminal alpha-helix mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
M286A | C-terminal alpha-helix mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
Q272A | active site mutant, pyocyanin production 1.4 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
R107A | mutant in KH-like motif, pyocyanin production 4.0 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
R111A | mutant in KH-like motif, pyocyanin production 0.3 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
R288A | active site mutant, pyocyanin production 5.2 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
R95A | mutant in KH-like motif, pyocyanin production 5.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
S273A | active site mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
S285A | active site mutant, pyocyanin production 5.0 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
S285W | active site mutant, pyocyanin production 5.6 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
W269A | C-terminal alpha-helix mutant, pyocyanin production 0.1 mg/l compared with 6.2 mg/l for wild-type | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | A0A0H2Z6F6 | - |
- |
Pseudomonas aeruginosa UCBPP-PA14 | A0A0H2Z6F6 | - |
- |
General Information | Comment | Organism |
---|---|---|
physiological function | the active site of the enzyme involves residues that extend further into the hydrophobic core of the protein, advocating for a lid-like movement of the two terminal helices. A unique C-terminal alpha-helical motif is important for the molecular function | Pseudomonas aeruginosa |