Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
cysteamine S-phosphate | pH 8.2, 30°C | Pseudomonas aeruginosa | |
2.5 | - |
acetoacetyl-CoA | pH 8.2, 30°C | Pseudomonas aeruginosa | |
4.4 | - |
S-ethyl-acetothioacetate | pH 8.2, 30°C | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoylacetyl-CoA + H2O | Pseudomonas aeruginosa | - |
2-aminobenzoylacetate + CoA | - |
? | |
2-aminobenzoylacetyl-CoA + H2O | Pseudomonas aeruginosa UCBPP-PA14 | - |
2-aminobenzoylacetate + CoA | - |
? | |
additional information | Pseudomonas aeruginosa | no substrates: anthraniloyl-CoA (or benzoyl-CoA), malonyl-CoA and octanoyl-CoA | ? | - |
? | |
additional information | Pseudomonas aeruginosa UCBPP-PA14 | no substrates: anthraniloyl-CoA (or benzoyl-CoA), malonyl-CoA and octanoyl-CoA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | A0A0H2Z6F6 | - |
- |
Pseudomonas aeruginosa UCBPP-PA14 | A0A0H2Z6F6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoylacetyl-CoA + H2O | - |
Pseudomonas aeruginosa | 2-aminobenzoylacetate + CoA | - |
? | |
2-aminobenzoylacetyl-CoA + H2O | - |
Pseudomonas aeruginosa UCBPP-PA14 | 2-aminobenzoylacetate + CoA | - |
? | |
acetoacetyl-CoA + H2O | - |
Pseudomonas aeruginosa | 3-oxobutanoic acid + CoA | - |
? | |
acetoacetyl-CoA + H2O | - |
Pseudomonas aeruginosa UCBPP-PA14 | 3-oxobutanoic acid + CoA | - |
? | |
cysteamine S-phosphate + H2O | - |
Pseudomonas aeruginosa | cysteamine + phosphate | - |
? | |
cysteamine S-phosphate + H2O | - |
Pseudomonas aeruginosa UCBPP-PA14 | cysteamine + phosphate | - |
? | |
additional information | no substrates: anthraniloyl-CoA (or benzoyl-CoA), malonyl-CoA and octanoyl-CoA | Pseudomonas aeruginosa | ? | - |
? | |
additional information | no substrates: anthraniloyl-CoA (or benzoyl-CoA), malonyl-CoA and octanoyl-CoA | Pseudomonas aeruginosa UCBPP-PA14 | ? | - |
? | |
S-ethyl-acetothioacetate + H2O | - |
Pseudomonas aeruginosa | 3-oxobutanoic acid + thioethanol | - |
? | |
S-ethyl-acetothioacetate + H2O | - |
Pseudomonas aeruginosa UCBPP-PA14 | 3-oxobutanoic acid + thioethanol | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
cysteamine S-phosphate | pH 8.2, 30°C | Pseudomonas aeruginosa | |
0.36 | - |
acetoacetyl-CoA | pH 8.2, 30°C | Pseudomonas aeruginosa | |
0.85 | - |
S-ethyl-acetothioacetate | pH 8.2, 30°C | Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
physiological function | in biosynthesis of the alkylquinolones 2-heptyl-3-hydroxy-4(1H)-quinolone and 2-heptyl-4(1H)-quinolone, PqsE acts as thioesterase, hydrolyzing the biosynthetic intermediate 2-aminobenzoylacetyl-coenzyme A to form 2-aminobenzoylacetate, the precursor of 2-heptyl-4(1H)-quinolone and 2-aminoacetophenone. The role of PqsE can be taken over to some extent by the broad-specificity thioesterase TesB. A pqsE mutant produces increased levels of 2,4-dihydroxyquinoline, resulting from intramolecular cyclization of 2-aminobenzoylacetyl-coenzyme A | Pseudomonas aeruginosa |