Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method. Crystals of wild type enzyme and mutants N37A, D52A, and T68A are obtained in conditions composed of 200 mM ammonium tartrate dibasic, 20% (w/v) PEG 3350, 100 mM Tris, pH 7.5; 200 mM lithium sulfate, 100 mM Tris-HCl, 30% (w/v) PEG 4000, 200 mM magnesium chloride, PEG 400, 100 mM HEPES, pH 7.5; 200 mM magnesium chloride, 14% (w/v) PEG 3350, 100 mM Tris, pH 6.0, respectively | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
D52A | the mutant has completely abolished activity | Streptococcus pneumoniae |
N37A | the mutant has completely abolished activity | Streptococcus pneumoniae |
T68A | the mutant shows a marked reduction in activity compared to the wild type enzyme | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | A0A0M3KL39 | - |
- |
Streptococcus pneumoniae ATCC BAA-334 | A0A0M3KL39 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap affinity column chromatography and gel filtration | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O | very low activity | Streptococcus pneumoniae | acetate + CoA | - |
? | |
acetyl-CoA + H2O | very low activity | Streptococcus pneumoniae ATCC BAA-334 | acetate + CoA | - |
? | |
arachidonyl-CoA + H2O | very low activity | Streptococcus pneumoniae | arachidonate + CoA | - |
? | |
butyryl-CoA + H2O | low activity | Streptococcus pneumoniae | butanoate + CoA | - |
? | |
butyryl-CoA + H2O | low activity | Streptococcus pneumoniae ATCC BAA-334 | butanoate + CoA | - |
? | |
decanoyl-CoA + H2O | highest activity | Streptococcus pneumoniae | decanoate + CoA | - |
? | |
dodecanoyl-CoA + H2O | high activity | Streptococcus pneumoniae | dodecanoate + CoA | - |
? | |
hexanoyl-CoA + H2O | - |
Streptococcus pneumoniae | hexanoate + CoA | - |
? | |
additional information | no activity with malonyl-CoA, palmitoyl-CoA, and stearoyl-CoA | Streptococcus pneumoniae | ? | - |
? | |
additional information | no activity with malonyl-CoA, palmitoyl-CoA, and stearoyl-CoA | Streptococcus pneumoniae ATCC BAA-334 | ? | - |
? | |
myristoyl-CoA + H2O | very low activity | Streptococcus pneumoniae | myristate + CoA | - |
? | |
myristoyl-CoA + H2O | very low activity | Streptococcus pneumoniae ATCC BAA-334 | myristate + CoA | - |
? | |
octanoyl-CoA + H2O | high activity | Streptococcus pneumoniae | octanoate + CoA | - |
? | |
palmitoyl-CoA + H2O | - |
Streptococcus pneumoniae | palmitate + CoA | - |
? | |
palmitoyl-CoA + H2O | - |
Streptococcus pneumoniae ATCC BAA-334 | palmitate + CoA | - |
? | |
phenylacetyl-CoA + H2O | low activity. The wild type enzyme displays a greater activity for phenylacetyl-CoA than very short and long chain saturated fatty acyl-CoA substrates | Streptococcus pneumoniae | phenylacetate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
PaaI | - |
Streptococcus pneumoniae |
PaaI thioesterase | - |
Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
phenylacetyl-CoA | wild type enzyme, at pH 7.6 and 21°C | Streptococcus pneumoniae | |
32.8 | - |
decanoyl-CoA | wild type enzyme, at pH 7.6 and 21°C | Streptococcus pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
72 | - |
phenylacetyl-CoA | wild type enzyme, at pH 7.6 and 21°C | Streptococcus pneumoniae | |
180 | - |
decanoyl-CoA | wild type enzyme, at pH 7.6 and 21°C | Streptococcus pneumoniae |