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Literature summary for 3.1.2.25 extracted from

  • Brian, S.
    Phenylacetyl coenzyme A hydrolase (1975), Methods Enzymol., 43, 482-487.
    View publication on PubMed
Search for more literature for 3.1.2.25

Inhibitors

Inhibitors Comment Organism Structure
additional information sensitive to SH group inhibitors Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.95
-
 phenylacetyl-CoA
-
 Penicillium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 gel filtration Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
-
-

Oxidation Stability

Oxidation Stability Organism
thiols are necessary during purification to prevent oxidation Penicillium chrysogenum

Purification (Commentary)

Purification (Comment) Organism
130fold Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl acetate + H2O hydrolyzes p-nitrophenyl acetate at the same rate as phenylacetyl-CoA or phenoxyacetylglycine Penicillium chrysogenum p-nitrophenol + acetate
-
 ?
phenoxyacetyl-CoA + H2O approximately 5fold higher activity than with phenylacetyl-CoA Penicillium chrysogenum phenoxyacetate + CoA
-
 ?
phenoxyacetylglycine + H2O hydrolyzes phenoxyacetylglycine at the same rate as phenylacetyl-CoA or p-nitrophenyl acetate Penicillium chrysogenum ?
-
 ?
phenylacetyl-CoA + H2O hydrolyzes phenylacetyl-CoA at the same rate as p-nitrophenyl acetate or phenoxyacetylglycine, approximately 5fold lower activity as with phenoxyacetyl-CoA Penicillium chrysogenum phenylacetate + CoA
-
 ?

Synonyms

Synonyms Comment Organism
phenylacetyl coenzyme A hydrolase
-
 Penicillium chrysogenum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 7.8
-
 Penicillium chrysogenum