Literature summary for 3.1.2.22 extracted from

Cho, S.K.; Hofmann, S.L.
pdf1, a palmitoyl protein thioesterase 1 ortholog in Schizosaccharomyces pombe: a yeast model of infantile Batten disease (2004), Eukaryot. Cell, 3, 302-310.

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis, enzyme is encoded in frame with a second gene coding for an indispensable dolichol pyrophosphatase, gene pdf1, strains bearing a deletion of the entire pdf1 open reading frame are nonviable, due to Dolpp1p deficiency, and are rescued by pdf1 expression plasmid, mutations only in the palmitoyl protein thioesterase 1 domain results in cells abnormally sensitive to sodium vanadate and elevated extracellular pH, the pdf1-deficient phenotype is complemented by the human PPT1 gene	Schizosaccharomyces pombe

Cloned (Comment)

Organism

DNA sequence determination and analysis, enzyme is encoded in frame with a second gene coding for an indispensable dolichol pyrophosphatase, gene pdf1, strains bearing a deletion of the entire pdf1 open reading frame are nonviable, due to Dolpp1p deficiency, and are rescued by pdf1 expression plasmid, mutations only in the palmitoyl protein thioesterase 1 domain results in cells abnormally sensitive to sodium vanadate and elevated extracellular pH, the pdf1-deficient phenotype is complemented by the human PPT1 gene

Schizosaccharomyces pombe

Inhibitors

Inhibitors	Comment	Organism	Structure
Sodium vanadate	pdf1 mutant	Schizosaccharomyces pombe

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
lysosome	-	Schizosaccharomyces pombe	5764	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	x * 31000, processed enzyme, SDS-PAGE, x * 101000, unprocessed enzyme, SDS-PAGE	Schizosaccharomyces pombe
101000	-	x * 31000, processed enzyme, SDS-PAGE, x * 101000, unprocessed enzyme, SDS-PAGE	Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Schizosaccharomyces pombe	PPT1 mutations cause severe neurodegenerative storage disorder, termed infantile Batten, in humans, Schizosaccharomyces pombe is a genetically tractable model for the sutdy of this disease due to evolutionary highly conserved gene and function	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	O59747	cosmid; isozyme PPT1. gene pdf1	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
proteolytic modification	enzyme contains 2 putative cleavage sites for the kex-related endopeptidase Krp1p, the precursor is proteolytically processed to form distinct Ppt1p and Dolpp1p domains, Arg354 is crucial for the processing	Schizosaccharomyces pombe

Reaction

Reaction	Comment	Organism	Reaction ID
palmitoyl[protein] + H2O = palmitate + protein	specific for long-chain thioesters of fatty acids from S-acylated residues in proteins, palmitoyl cysteine and palmitoyl-CoA	Schizosaccharomyces pombe	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl 6-S-palmitoyl-6-thio-beta-D-glucoside + H2O	artificial substrate	Schizosaccharomyces pombe	4-methylumbelliferyl 6-thio-beta-D-glucoside + palmitate	-	?
additional information	PPT1 mutations cause severe neurodegenerative storage disorder, termed infantile Batten, in humans, Schizosaccharomyces pombe is a genetically tractable model for the sutdy of this disease due to evolutionary highly conserved gene and function	Schizosaccharomyces pombe	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 31000, processed enzyme, SDS-PAGE, x * 101000, unprocessed enzyme, SDS-PAGE	Schizosaccharomyces pombe

Synonyms

Synonyms	Comment	Organism
palmitoyl protein thioesterase 1	-	Schizosaccharomyces pombe
PPT1	-	Schizosaccharomyces pombe