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Literature summary for 3.1.2.20 extracted from

  • Wu, H.; Liang, J.; Gou, L.; Wu, Q.; Liang, W.J.; Zhou, X.; Bruce, I.J.; Deng, Z.; Wang, Z.
    Recycling of overactivated acyls by a type II thioesterase during calcimycin biosynthesis in Streptomyces chartreusis NRRL 3882 (2018), Appl. Environ. Microbiol., 84, e00587-18 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.2.20

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Streptomyces chartreusis
Fe2+ almost complete inhibition at 1 mM Streptomyces chartreusis
Mg2+
-
 Streptomyces chartreusis
Mn2+ almost complete inhibition at 10 mM Streptomyces chartreusis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.141
-
 palmitoyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.188
-
 malonyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.19
-
 butyryl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.2
-
 acetyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.216
-
 benzoyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.67
-
 cezomycin-CoA at pH 7.5 and 30°C Streptomyces chartreusis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Zn2+ Streptomyces chartreusis

Organism

Organism UniProt Comment Textmining
Streptomyces chartreusis
-
-
-
Streptomyces chartreusis NRRL 3882
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and HisTrap column chromatography Streptomyces chartreusis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O 23% activity compared to cezomycin-CoA Streptomyces chartreusis CoA + acetate
-
 ?
acetyl-CoA + H2O 23% activity compared to cezomycin-CoA Streptomyces chartreusis NRRL 3882 CoA + acetate
-
 ?
benzoyl-CoA + H2O 16% activity compared to cezomycin-CoA Streptomyces chartreusis CoA + benzoate
-
 ?
benzoyl-CoA + H2O 16% activity compared to cezomycin-CoA Streptomyces chartreusis NRRL 3882 CoA + benzoate
-
 ?
butyryl-CoA + H2O 24% activity compared to cezomycin-CoA Streptomyces chartreusis CoA + butyrate
-
 ?
cezomycin-CoA + H2O 100% activity Streptomyces chartreusis CoA + cezomycin
-
 ?
malonyl-CoA + H2O 20% activity compared to cezomycin-CoA Streptomyces chartreusis CoA + malonate
-
 ?
malonyl-CoA + H2O 20% activity compared to cezomycin-CoA Streptomyces chartreusis NRRL 3882 CoA + malonate
-
 ?
additional information no activity with 3-S-N-acetylcysteamine. The enzyme is not involved in the release of calcimycin from the polyketide megasynthase Streptomyces chartreusis ?
-
 ?
additional information no activity with 3-S-N-acetylcysteamine. The enzyme is not involved in the release of calcimycin from the polyketide megasynthase Streptomyces chartreusis NRRL 3882 ?
-
 ?
palmitoyl-CoA + H2O 1% activity compared to cezomycin-CoA Streptomyces chartreusis CoA + palmitate
-
 ?
palmitoyl-CoA + H2O 1% activity compared to cezomycin-CoA Streptomyces chartreusis NRRL 3882 CoA + palmitate
-
 ?

Subunits

Subunits Comment Organism
? x * 27900, SDS-PAGE Streptomyces chartreusis

Synonyms

Synonyms Comment Organism
CalG
-
 Streptomyces chartreusis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0009
-
 palmitoyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.015
-
 benzoyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.017
-
 malonyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.021
-
 acetyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.021
-
 butyryl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.3
-
 cezomycin-CoA at pH 7.5 and 30°C Streptomyces chartreusis

General Information

General Information Comment Organism
metabolism the enzyme prevents the accumulation of cezomycin-CoA when intracellular nitrogen is limited Streptomyces chartreusis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0062
-
 palmitoyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.011
-
 butyryl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.071
-
 benzoyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.09
-
 malonyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.105
-
 acetyl-CoA at pH 7.5 and 30°C Streptomyces chartreusis
0.442
-
 cezomycin-CoA at pH 7.5 and 30°C Streptomyces chartreusis