Literature summary for 3.1.2.20 extracted from

Willis, M.A.; Zhuang, Z.; Song, F.; Howard, A.; Dunaway-Mariano, D.; Herzberg, O.
Structure of YciA from Haemophilus influenzae (HI0827), a hexameric broad specificity acyl-coenzyme A thioesterase (2008), Biochemistry, 47, 2797-2805.

Search for more literature for 3.1.2.20

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)	Haemophilus influenzae

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method	Haemophilus influenzae

Protein Variants

Protein Variants	Comment	Organism
D44A	putative catalytic residue	Haemophilus influenzae
N29A	involved in substrate binding	Haemophilus influenzae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0042	-	lauroyl-CoA	N29A mutant protein	Haemophilus influenzae
0.0066	-	lauroyl-CoA	wild-type protein	Haemophilus influenzae
0.013	-	isobutyryl-CoA	wild-type protein	Haemophilus influenzae
0.092	-	isobutyryl-CoA	N29A mutant protein	Haemophilus influenzae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
17000	-	6 * 17000, crystal structure analysis	Haemophilus influenzae

Organism

Organism	UniProt	Comment	Textmining
Haemophilus influenzae	P44886	stain Rd KW20	-

Purification (Commentary)

Purification (Comment)	Organism
-	Haemophilus influenzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
butyryl-CoA + H2O	-	Haemophilus influenzae	butanoate + CoA	-	?
butyrylpanthetheine phosphate + H2O	-	Haemophilus influenzae	pantetheine phosphate + butyrate	-	?
isobutyryl-CoA + H2O	-	Haemophilus influenzae	CoA + isobutyrate	-	?
lauroyl-CoA + H2O	-	Haemophilus influenzae	CoA + lauroate	-	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 17000, crystal structure analysis	Haemophilus influenzae

Synonyms

Synonyms	Comment	Organism
acyl-Coenzyme A thioesterase	-	Haemophilus influenzae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	D44A mutant protein: value below 0.001 s-1 for isobutyryl-CoA and lauroyl-CoA	Haemophilus influenzae
0.08	-	butyrylpantetheine phosphate	wild-type protein	Haemophilus influenzae
2.34	-	isobutyryl-CoA	N29A mutant protein	Haemophilus influenzae
2.57	-	lauroyl-CoA	N29A mutant protein	Haemophilus influenzae
6.7	-	butyryl-CoA	wild-type protein	Haemophilus influenzae
9.2	-	lauroyl-CoA	wild-type protein	Haemophilus influenzae
138	-	isobutyryl-CoA	wild-type protein	Haemophilus influenzae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.1	-	butyrylpantetheine phosphate	wild-type protein	Haemophilus influenzae
25	-	isobutyryl-CoA	N29A mutant protein	Haemophilus influenzae
100	-	butyryl-CoA	wild-type protein	Haemophilus influenzae
610	-	lauroyl-CoA	N29A mutant protein	Haemophilus influenzae
1400	-	lauroyl-CoA	wild-type protein	Haemophilus influenzae
11000	-	isobutyryl-CoA	wild-type protein	Haemophilus influenzae

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Release 2023.1 (January 2023)