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Literature summary for 3.1.2.20 extracted from

  • Guo, Z.F.; Sun, Y.; Zheng, S.; Guo, Z.
    Preferential hydrolysis of aberrant iIntermediates by the type II thioesterase in Escherichia coli nonribosomal enterobactin synthesis: substrate specificities and mutagenic studies on the active-site residues (dagger) (2009), Biochemistry, 48, 1712-1722.
    View publication on PubMed
Search for more literature for 3.1.2.20

Activating Compound

Activating Compound Comment Organism Structure
additional information EntH active site consists of Gln48, Gly55, Glu63, Thr64, Ser67, Met68, and Gly82 Escherichia coli K-12

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed with a C-terminal hexahistidine tag Escherichia coli K-12

Protein Variants

Protein Variants Comment Organism
E63A no catalytic activity toward salicylyl-CoA and 2,3-dihydroxybenzoyl-CoA Escherichia coli K-12
E63D no catalytic activity toward salicylyl-CoA and 2,3-dihydroxybenzoyl-CoA Escherichia coli K-12
E63Q no catalytic activity toward salicylyl-CoA and 2,3-dihydroxybenzoyl-CoA Escherichia coli K-12
H54A 229fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
M68A 130fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
Q48A no catalytic activity toward salicylyl-CoA and 2,3-dihydroxybenzoyl-CoA Escherichia coli K-12
Q48N 290fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
S67A 140fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
S67C decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12
T64S 13fold decrease in catalytic efficiency toward salicylyl-CoA Escherichia coli K-12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00425
-
 palmitoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.0375
-
 2,3-dihydroxybenzoyl-CoA mutant H54A, at room temperature Escherichia coli K-12
0.116
-
 2,3-dihydroxybenzoyl-EntB wild-type, at room temperature Escherichia coli K-12
0.161
-
 2,3-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.162
-
 salicylyl-CoA mutant H54A, at room temperature Escherichia coli K-12
0.176
-
 salicylyl-CoA wild-type, at room temperature Escherichia coli K-12
0.19
-
 4-hydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.196
-
 salicylyl-CoA mutant S67C, at room temperature Escherichia coli K-12
0.212
-
 3,4-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.219
-
 2,4-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.256
-
 3,5-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.263
-
 salicylyl-CoA mutant S67A, at room temperature Escherichia coli K-12
0.265
-
 3-hydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.272
-
 salicylyl-EntB wild-type, at room temperature Escherichia coli K-12
0.329
-
 2,3-dihydroxybenzoyl-CoA mutant S67A, at room temperature Escherichia coli K-12
0.373
-
 salicylyl-CoA mutant M68A, at room temperature Escherichia coli K-12
0.383
-
 2,3-dihydroxybenzoyl-CoA mutant T64S, at room temperature Escherichia coli K-12
0.454
-
 2,3-dihydroxybenzoyl-CoA mutant S67C, at room temperature Escherichia coli K-12
0.475
-
 benzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.505
-
 2,3-dihydroxybenzoyl-CoA mutant Q48N, at room temperature Escherichia coli K-12
0.672
-
 salicylyl-CoA mutant T64S, at room temperature Escherichia coli K-12
0.729
-
 salicylyl-CoA mutant Q48N, at room temperature Escherichia coli K-12
1.42
-
 2,3-dihydroxybenzoyl-CoA mutant M68A, at room temperature Escherichia coli K-12

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54400
-
 gel filtration Escherichia coli K-12

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by metal chelating chromatography and gel filtration Escherichia coli K-12

Storage Stability

Storage Stability Organism
-20°C, PBS buffer, pH 7.4, 10% glycerol Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3-dihydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 2,3-dihydroxybenzoate
-
 ?
2,3-dihydroxybenzoyl-EntB + H2O
-
 Escherichia coli K-12 EntB + 2,3-dihydroxybenzoate
-
 ?
2,4-dihydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 2,4-dihydroxybenzoate
-
 ?
3,4-dihydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 3,4-dihydroxybenzoate
-
 ?
3,5-dihydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 3,5-dihydroxybenzoate
-
 ?
3-hydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 3-hydroxybenzoate
-
 ?
4-hydroxybenzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + 4-hydroxybenzoate
-
 ?
benzoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + benzoate
-
 ?
additional information EntH is unable to recognize acetyl-CoA, salicylyl-N-acetylcystamine or 2,3-dihydroxybenzoyl-N-acetylcystamine Escherichia coli K-12 ?
-
 ?
palmitoyl-CoA + H2O
-
 Escherichia coli K-12 CoA + palmitate
-
 ?
salicylyl-CoA + H2O
-
 Escherichia coli K-12 CoA + salicylate
-
 ?
salicylyl-EntB + H2O
-
 Escherichia coli K-12 EntB + salicylate
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Escherichia coli K-12

Synonyms

Synonyms Comment Organism
EntH
-
 Escherichia coli K-12
TEII
-
 Escherichia coli K-12
type II thioesterase
-
 Escherichia coli K-12

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00217
-
 2,3-dihydroxybenzoyl-CoA mutant H54A, at room temperature Escherichia coli K-12
0.00333
-
 2,3-dihydroxybenzoyl-CoA mutant S67C, at room temperature Escherichia coli K-12
0.0112
-
 2,3-dihydroxybenzoyl-CoA mutant S67A, at room temperature Escherichia coli K-12
0.0155
-
 2,3-dihydroxybenzoyl-CoA mutant Q48N, at room temperature Escherichia coli K-12
0.019
-
 salicylyl-CoA mutant H54A, at room temperature Escherichia coli K-12
0.0342
-
 2,4-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.0507
-
 salicylyl-CoA mutant S67A, at room temperature Escherichia coli K-12
0.051
-
 salicylyl-CoA mutant S67C, at room temperature Escherichia coli K-12
0.0678
-
 salicylyl-CoA mutant Q48N, at room temperature Escherichia coli K-12
0.0778
-
 salicylyl-CoA mutant M68A, at room temperature Escherichia coli K-12
0.138
-
 2,3-dihydroxybenzoyl-CoA mutant T64S, at room temperature Escherichia coli K-12
0.14
-
 2,3-dihydroxybenzoyl-EntB wild-type, at room temperature Escherichia coli K-12
0.154
-
 palmitoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.207
-
 4-hydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.23
-
 3,4-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.372
-
 2,3-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
0.533
-
 2,3-dihydroxybenzoyl-CoA mutant M68A, at room temperature Escherichia coli K-12
1.38
-
 salicylyl-CoA mutant T64S, at room temperature Escherichia coli K-12
4.63
-
 salicylyl-CoA wild-type, at room temperature Escherichia coli K-12
4.83
-
 salicylyl-EntB wild-type, at room temperature Escherichia coli K-12
7.45
-
 3,5-dihydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
10.3
-
 3-hydroxybenzoyl-CoA wild-type, at room temperature Escherichia coli K-12
13.9
-
 benzoyl-CoA wild-type, at room temperature Escherichia coli K-12