Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.2.20 extracted from

  • Zhou, Y.; Meng, Q.; You, D.; Li, J.; Chen, S.; Ding, D.; Zhou, X.; Zhou, H.; Bai, L.; Deng, Z.
    Selective removal of aberrant extender units by a type II thioesterase for efficient FR-008/candicidin biosynthesis in Streptomyces sp. strain FR-008 (2008), Appl. Environ. Microbiol., 74, 7235-7242.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.2.20

Activating Compound

Activating Compound Comment Organism Structure
additional information overexpression of FscTE under the strong constitutive ermE*p promoter in the wild-type strain, does not suppress FR-008/candicidin formation Streptomyces sp.

Application

Application Comment Organism
degradation TEII can maintain effective polyketide biosynthesis by selectively removing the nonelongatable residues bound to acyl carrier proteins Streptomyces sp.

Cloned(Commentary)

Cloned (Comment) Organism
FscTE cloned into vector pET28a and expressed in Escherichia coli BL21(DE3)/pLysE Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
additional information deletion of FscTE reduces approximately 90% of the FR-008/candicidin production, while the production level is well restored when FscTE is added back to the mutant in trans. Successful complementation of the FscTE mutant with FscTE and tylO, whereas no complementation with nonribosomal peptide synthetase TEII tycF and srfAD Streptomyces sp.
S129A introduced into mutant ZYJ-4 (shows only 10% of the original FR-008/candicidin production) provides no contribution to the restoration of FR-008/candicidin titer Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
32
-
 propionyl-S-n-acetylcysteamine at pH 7.4, 30°C Streptomyces sp.
33.4
-
 acetyl-S-N-acetylcysteamine at pH 7.4, 30°C Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33855
-
 1 * 33855, sequence analysis Streptomyces sp.
35000
-
 1 * 35000, SDS-PAGE Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. FR-008
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by Ni2+ affinity column chromatography Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-S-N-acetylcysteamine efficient hydrolysis, remarkable preferences for acylthioesters Streptomyces sp. N-acetylcysteamine + acetate
-
 ?
acetyl-S-N-acetylcysteamine efficient hydrolysis, remarkable preferences for acylthioesters Streptomyces sp. FR-008 N-acetylcysteamine + acetate
-
 ?
malonyl-S-N-acetylcysteamine limited hydrolysis Streptomyces sp. N-acetylcysteamine + malonate
-
 ?
malonyl-S-N-acetylcysteamine limited hydrolysis Streptomyces sp. FR-008 N-acetylcysteamine + malonate
-
 ?
methylmalonyl-S-N-acetylcysteamine limited hydrolysis Streptomyces sp. N-acetylcysteamine + methylmalonate
-
 ?
methylmalonyl-S-N-acetylcysteamine limited hydrolysis Streptomyces sp. FR-008 N-acetylcysteamine + methylmalonate
-
 ?
additional information p-aminobenzoyl-S-N-acetylcysteamine is no proper substrate Streptomyces sp. ?
-
 ?
additional information p-aminobenzoyl-S-N-acetylcysteamine is no proper substrate Streptomyces sp. FR-008 ?
-
 ?
propionyl-S-N-acetylcysteamine efficient hydrolysis, remarkable preferences for acylthioesters Streptomyces sp. N-acetylcysteamine + propionate
-
 ?
propionyl-S-N-acetylcysteamine efficient hydrolysis, remarkable preferences for acylthioesters Streptomyces sp. FR-008 N-acetylcysteamine + propionate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 35000, SDS-PAGE Streptomyces sp.
monomer 1 * 33855, sequence analysis Streptomyces sp.

Synonyms

Synonyms Comment Organism
FscTE
-
 Streptomyces sp.
TEII
-
 Streptomyces sp.
TEII associated with the FR-008/candicidin gene cluster
-
 Streptomyces sp.
type II thioesterase
-
 Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.193
-
 acetyl-S-N-acetylcysteamine at pH 7.4, 30°C Streptomyces sp.
1.82
-
 propionyl-S-n-acetylcysteamine at pH 7.4, 30°C Streptomyces sp.