Literature summary for 3.1.2.20 extracted from

Zhuang, Z.; Song, F.; Zhao, H.; Li, L.; Cao, J.; Eisenstein, E.; Herzberg, O.; Dunaway-Mariano, D.
Divergence of function in the hot dog fold enzyme superfamily: the bacterial thioesterase YciA (2008), Biochemistry, 47, 2789-2796.

Cloned(Commentary)

Cloned (Comment)	Organism
the gene encoding YciA is amplified by PCR and expressed in Escherichia coli strain BL21 (DE3)	Escherichia coli
the gene encoding YciA is amplified by PCR and expressed in Escherichia coli strain BL21 (DE3)	Haemophilus influenzae

General Stability

General Stability	Organism
YciA is a highly soluble, robust protein	Haemophilus influenzae
YciA tends to precipitate at high concentrations	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism
4-hydroxyphenacyl-CoA	-	Escherichia coli
4-hydroxyphenacyl-CoA	-	Haemophilus influenzae
CoA	strong feedback inhibitor, product inhibition	Escherichia coli
CoA	strong feedback inhibitor, product inhibition	Haemophilus influenzae
hexyl-CoA	-	Escherichia coli
hexyl-CoA	-	Haemophilus influenzae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.0016	-	phenylacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0019	-	n-octanoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0021	-	tiglyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0025	-	myristoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0035	-	n-butyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0037	-	3-hydroxyphenylacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0041	-	n-hexanoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0042	-	linoleoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0045	-	n-decanoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0047	-	acetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0059	-	crotonyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.006	-	propionyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.006	-	lauroyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0064	-	palmitoleoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0076	-	4-hydroxyphenylacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.0081	-	glutaryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.009	-	beta-methylcrotonyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.01	-	stearoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.011	-	oleoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.011	-	oleoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.013	-	isobutyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.013	-	n-decanoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.014	-	isovaleryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.015	-	isobutyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.016	-	phenylacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.017	-	palmitoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.02	-	succinyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.02	-	lauroyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.02	-	DL-3-hydroxy-3-methylglutaryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.025	-	4-hydroxybenzoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.031	-	DL-3-hydroxybutyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.037	-	acetoacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.039	-	arachidoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.068	-	n-butyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.14	-	malonyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.167	-	acetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.17	-	methylmalonyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.29	-	beta-methylcrotonyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.8	-	n-butyryl-pantetheine phosphate	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
1.4	-	n-butyryl-pantetheine phosphate	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
33	-	palmitoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Escherichia coli	5737	-
cytoplasm	-	Haemophilus influenzae	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
15160	-	His-tagged YciA protein, determined by electrospray ionization mass spectrometry	Escherichia coli
16630	-	His-tagged YciA protein, determined by electrospray ionization mass spectrometry, N-terminal methionine is removed	Haemophilus influenzae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
4-Hydroxybenzoyl-CoA + H2O	Escherichia coli	-	4-Hydroxybenzoate + CoA	-	?
4-Hydroxybenzoyl-CoA + H2O	Haemophilus influenzae	-	4-Hydroxybenzoate + CoA	-	?
acetyl-CoA + H2O	Escherichia coli	-	CoA + acetate	-	?
acetyl-CoA + H2O	Haemophilus influenzae	-	CoA + acetate	-	?
additional information	Escherichia coli	the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites	?	-	?
additional information	Haemophilus influenzae	the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites	?	-	?
additional information	Haemophilus influenzae	the substrate activity of propionyl-s-N-acetylcysteine is negligible and that of n-butyryl-pantetheine phosphate low, it is evident YciA does not target acylated holoacyl carrier proteins or other acylated proteins as substrates	?	-	?
additional information	Escherichia coli	YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis	?	-	?
additional information	Haemophilus influenzae	YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A8Z0	-	-
Haemophilus influenzae	P44886	-	-

Purification (Commentary)

Purification (Comment)	Organism
Cells are harvested by centrifugation and suspended in lysis buffer, passed through a French press and centrifuged. The supernatant is loaded onto a Ni-NTA agarose column. The column is washed with a buffer containing 50 mM NaH2PO4, 300 mM NaCl, 50 mM imidazole and 1 mM dithiothreitol, and then the YciA is eluted with an imidazole gradient up to 250 mM imidazole. The pooled and concentrated fractions are chromatographed on a size exclusion column using a buffer containing 50 mM NaH2PO4, 50 mM NaCl, 10 mM imidazole and 1 mM dithiothreitol.	Escherichia coli
Cells are harvested by centrifugation, and the cell pellet is suspended in HEPES buffer containing 2 mM DTT and 0.2 mM phenylmethanesulfonyl fluoride. The cells are lysed in a French press and the lysate is centrifuged. The supernatant is loaded onto a DEAE Sepharose Fast Flow column equilibrated with HEPES buffer. A linear gradient of KCl in 50 mM Hepes is used to elute the protein. Desired fractions are pooled, concentrated and subjected to ammonium sulfate fractionation. The 40-60% ammonium sulfate fraction is centrifuged, the protein pellet is dissolved, loaded onto a size exclusion column and eluted with 10 mM HEPES containing 0.15 M KCl.	Haemophilus influenzae

Purification (Comment)

Organism

Cells are harvested by centrifugation and suspended in lysis buffer, passed through a French press and centrifuged. The supernatant is loaded onto a Ni-NTA agarose column. The column is washed with a buffer containing 50 mM NaH2PO4, 300 mM NaCl, 50 mM imidazole and 1 mM dithiothreitol, and then the YciA is eluted with an imidazole gradient up to 250 mM imidazole. The pooled and concentrated fractions are chromatographed on a size exclusion column using a buffer containing 50 mM NaH2PO4, 50 mM NaCl, 10 mM imidazole and 1 mM dithiothreitol.

Escherichia coli

Cells are harvested by centrifugation, and the cell pellet is suspended in HEPES buffer containing 2 mM DTT and 0.2 mM phenylmethanesulfonyl fluoride. The cells are lysed in a French press and the lysate is centrifuged. The supernatant is loaded onto a DEAE Sepharose Fast Flow column equilibrated with HEPES buffer. A linear gradient of KCl in 50 mM Hepes is used to elute the protein. Desired fractions are pooled, concentrated and subjected to ammonium sulfate fractionation. The 40-60% ammonium sulfate fraction is centrifuged, the protein pellet is dissolved, loaded onto a size exclusion column and eluted with 10 mM HEPES containing 0.15 M KCl.

Haemophilus influenzae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
3-hydroxyphenylacetyl-CoA + H2O	-	Haemophilus influenzae	CoA + 3-hydroxyphenylacetate	-	?
4-Hydroxybenzoyl-CoA + H2O	-	Escherichia coli	4-Hydroxybenzoate + CoA	-	?
4-Hydroxybenzoyl-CoA + H2O	-	Haemophilus influenzae	4-Hydroxybenzoate + CoA	-	?
4-hydroxyphenylacetyl-CoA + H2O	-	Haemophilus influenzae	CoA + 4-hydroxyphenylacetate	-	?
acetoacetyl-CoA + H2O	-	Haemophilus influenzae	CoA + acetoacetate	-	?
acetyl-CoA + H2O	-	Escherichia coli	CoA + acetate	-	?
acetyl-CoA + H2O	-	Haemophilus influenzae	CoA + acetate	-	?
arachidoyl-CoA + H2O	-	Haemophilus influenzae	CoA + arachidate	-	?
beta-methylcrotonyl-CoA + H2O	-	Escherichia coli	CoA + beta-methylcrotonate	-	?
beta-methylcrotonyl-CoA + H2O	-	Haemophilus influenzae	CoA + beta-methylcrotonate	-	?
crotonyl-CoA + H2O	-	Haemophilus influenzae	CoA + crotonate	-	?
DL-3-hydroxy-3-methylglutaryl-CoA + H2O	-	Haemophilus influenzae	CoA + 3-hydroxy-3-methylglutarate	-	?
DL-3-hydroxybutyryl-CoA + H2O	-	Haemophilus influenzae	CoA + ?	-	?
glutaryl-CoA + H2O	-	Haemophilus influenzae	CoA + glutarate	-	?
hexanoyl-CoA + H2O	-	Haemophilus influenzae	CoA + hexanoate	-	?
isobutyryl-CoA + H2O	-	Escherichia coli	CoA + isobutyrate	-	?
isobutyryl-CoA + H2O	-	Haemophilus influenzae	CoA + isobutyrate	-	?
isovaleryl-CoA + H2O	-	Haemophilus influenzae	CoA + isovalerate	-	?
lauroyl-CoA + H2O	-	Escherichia coli	CoA + laurate	-	?
lauroyl-CoA + H2O	-	Haemophilus influenzae	CoA + laurate	-	?
linoleoyl-CoA + H2O	-	Haemophilus influenzae	CoA + linolate	-	?
malonyl-CoA + H2O	-	Haemophilus influenzae	CoA + malonate	-	?
methylmalonyl-CoA + H2O	-	Haemophilus influenzae	CoA + methylmalonate	-	?
additional information	the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites	Escherichia coli	?	-	?
additional information	the long, medium, and short chain fatty acyl-CoAs are very good substrates as are the small acyl-CoA metabolites	Haemophilus influenzae	?	-	?
additional information	the substrate activity of propionyl-s-N-acetylcysteine is negligible and that of n-butyryl-pantetheine phosphate low, it is evident YciA does not target acylated holoacyl carrier proteins or other acylated proteins as substrates	Haemophilus influenzae	?	-	?
additional information	YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis	Escherichia coli	?	-	?
additional information	YciA is responsible for the efficient, indiscriminant CoA-regulated hydrolysis of cellular acyl-CoA thioesters in a wide range of bacteria and hypothesize that this activity may support membrane biogenesis	Haemophilus influenzae	?	-	?
myristoyl-CoA + H2O	-	Haemophilus influenzae	CoA + myristate	-	?
n-butyryl-CoA + H2O	-	Escherichia coli	CoA + n-butyrate	-	?
n-butyryl-CoA + H2O	-	Haemophilus influenzae	CoA + n-butyrate	-	?
n-butyryl-pantetheine phosphate + H2O	-	Escherichia coli	?	-	?
n-butyryl-pantetheine phosphate + H2O	-	Haemophilus influenzae	?	-	?
n-decanoyl-CoA + H2O	-	Escherichia coli	CoA + n-decanoate	-	?
n-decanoyl-CoA + H2O	-	Haemophilus influenzae	CoA + n-decanoate	-	?
n-octanoyl-CoA + H2O	-	Haemophilus influenzae	CoA + n-octanoate	-	?
oleoyl-CoA + H2O	-	Escherichia coli	CoA + oleate	-	?
oleoyl-CoA + H2O	-	Haemophilus influenzae	CoA + oleate	-	?
palmitoleoyl-CoA + H2O	-	Haemophilus influenzae	CoA + palmitoleoate	-	?
palmitoyl-CoA + H2O	-	Escherichia coli	CoA + palmitate	-	?
palmitoyl-CoA + H2O	-	Haemophilus influenzae	CoA + palmitate	-	?
phenylacetyl-CoA + H2O	-	Escherichia coli	CoA + phenylacetate	-	?
phenylacetyl-CoA + H2O	-	Haemophilus influenzae	CoA + phenylacetate	-	?
propionyl-CoA + H2O	-	Haemophilus influenzae	CoA + propionate	-	?
propionyl-s-N-acetylcysteamine + H2O	-	Haemophilus influenzae	CoA + ?	-	?
stearoyl-CoA + H2O	-	Haemophilus influenzae	CoA + stearate	-	?
succinyl-CoA + H2O	-	Haemophilus influenzae	CoA + succinate	-	?
tiglyl-CoA + H2O	-	Haemophilus influenzae	CoA + (2E)-2-methylbut-2-enoic acid	-	?

Subunits

Subunits	Comment	Organism
homohexamer	determined by using equilibrium sedimentation techniques	Haemophilus influenzae
homohexamer	YciA determined by using equilibrium sedimentation techniques	Escherichia coli

Synonyms

Synonyms	Comment	Organism
acyl-CoA hydrolase	-	Escherichia coli
acyl-CoA hydrolase	-	Haemophilus influenzae
thioesterase	-	Escherichia coli
thioesterase	-	Haemophilus influenzae
YciA	-	Escherichia coli
YciA	-	Haemophilus influenzae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Escherichia coli
25	-	assay at	Haemophilus influenzae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
additional information	-	propionyl-S-n-acetylcysteamine	absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured, Kcat is above 0.0001	Haemophilus influenzae
0.0062	-	4-hydroxybenzoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.078	-	4-hydroxybenzoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.082	-	n-butyryl-pantetheine phosphate	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.1	-	beta-methylcrotonyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.27	-	arachidoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.54	-	beta-methylcrotonyl-CoA	absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured	Haemophilus influenzae
0.55	-	acetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
0.77	-	stearoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.8	-	n-butyrylpantethiene phosphate	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
0.92	-	crotonyl-CoA	absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured	Haemophilus influenzae
1.1	-	methylmalonyl-CoA	absorbance of 5-thio-2-nitrobenzoate formed by reaction of 5,5'-dithiobis(2-nitrobenzoic acid) is measured	Haemophilus influenzae
1.3	-	acetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
1.7	-	oleoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
2	-	linoleoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
2.6	-	tiglyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
2.7	-	palmitoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
3.3	-	malonyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
3.7	-	palmitoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
4.6	-	n-octanoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
5.7	-	palmitoleoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
6.7	-	n-butyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
6.9	-	myristoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
8.3	-	DL-3-hydroxybutyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
8.9	-	DL-3-hydroxy-3-methylglutaryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
9.2	-	lauroyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
9.4	-	phenylacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
9.5	-	isobutyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
9.6	-	lauroyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
13	-	oleoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
13	-	n-decanoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
15	-	hexanoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
17	-	n-decanoyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Escherichia coli
22	-	n-butyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
25	-	propionyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
30	-	isovaleryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
35	-	acetoacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
75	-	4-hydroxyphenylacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
100	-	glutaryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
130	-	phenylacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
140	-	isobutyryl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
140	-	3-hydroxyphenylacetyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae
190	-	succinyl-CoA	pH 7.5, 25°C, determined using the 5,5'-dithiobis(2-nitrobenzoic acid) spectrophotometric assay	Haemophilus influenzae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Escherichia coli
7.5	-	assay at	Haemophilus influenzae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
additional information	-	additional information	noncompetitive inhibition of YciA-catalyzed isobutyryl-CoA hydrolysis is observed which defined a Kii of 0.0047 mM (Vmax altered) and a Kis of 0.33 microM (Vmax/Km altered)	Haemophilus influenzae
0.0019	-	hexyl-CoA	-	Haemophilus influenzae
0.0054	-	hexyl-CoA	-	Escherichia coli
0.0058	-	4-hydroxyphenacyl-CoA	-	Haemophilus influenzae