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Literature summary for 3.1.2.20 extracted from

  • Shahi, P.; Kumar, I.; Sharma, R.; Sanger, S.; Jolly, R.S.
    Characterization of a novel long-chain acyl-CoA thioesterase from Alcaligenes faecalis (2006), FEBS J., 273, 2374-2387.
    View publication on PubMed
Search for more literature for 3.1.2.20

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate 1 mM, complete inhibition Alcaligenes faecalis
dimethyl (2-hydroxy-5-nitrobenzyl) sulfonium bromide 1 mM, 11% inhibition Alcaligenes faecalis
dithiothreitol 1 mM, 20% inhibition Alcaligenes faecalis
N-bromosuccinimide 1 mM, complete inhibition Alcaligenes faecalis
p-chloromercuribenzoate 1 mM, 12% inhibition Alcaligenes faecalis
picrylsulfonic acid 1 mM, 11% inhibition Alcaligenes faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00266
-
 myristoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
0.00284
-
 cis-vaccenoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
0.00339
-
 myristoleoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
0.00348
-
 lauroyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
0.00359
-
 stearoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
0.00385
-
 palmitoyl-CoA 20°C, pH 7.6 Alcaligenes faecalis
0.0039
-
 palmitoleoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
0.00411
-
 palmitoyl-CoA 37°C, pH 7.6 Alcaligenes faecalis
0.00551
-
 cis-vaccenoyl-CoA 20°C, pH 7.6 Alcaligenes faecalis
0.0072
-
 palmitoleoyl-CoA 20°C, pH 7.6 Alcaligenes faecalis

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface exclusively associated with Alcaligenes faecalis 9986
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 2 * 22000 Alcaligenes faecalis

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alcaligenes faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-vaccenoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + cis-vaccenic acid
-
 ?
lauroyl-CoA + H2O
-
 Alcaligenes faecalis CoA + lauric acid
-
 ?
additional information long-chain acyl-CoAs are better substrates for thioesterase than p-nitrophenyl esters Alcaligenes faecalis ?
-
 ?
myristoleoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + myristoleic acid
-
 ?
myristoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + myristic acid
-
 ?
p-nitrophenyl acetate + H2O 30.14% of the activity with p-nitrophenyl propanoate Alcaligenes faecalis p-nitrophenol + acetate
-
 ?
p-nitrophenyl butanoate + H2O 51% of the activity with p-nitrophenyl propanoate Alcaligenes faecalis p-nitrophenol + butanoate
-
 ?
p-nitrophenyl dodecanoate + H2O 8.7% of the activity with p-nitrophenyl propanoate Alcaligenes faecalis p-nitrophenol + dodecanoate
-
 ?
p-nitrophenyl hexanoate + H2O 33% of the activity with p-nitrophenyl propanoate Alcaligenes faecalis p-nitrophenol + hexanoate
-
 ?
p-nitrophenyl propanoate + H2O
-
 Alcaligenes faecalis p-nitrophenol + propanoate
-
 ?
palmitoleoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + palmitoleic acid
-
 ?
palmitoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + palmitic acid
-
 ?
stearoyl-CoA + H2O
-
 Alcaligenes faecalis CoA + stearate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 22000 Alcaligenes faecalis

Synonyms

Synonyms Comment Organism
long-chain acyl-CoA thioesterase
-
 Alcaligenes faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Alcaligenes faecalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 75 25°C: about 65% of maximal activity, 75°C: about 60% of maximal activity Alcaligenes faecalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 3 h, no loss of activity Alcaligenes faecalis
60
-
 3 h, about 5% loss of activity Alcaligenes faecalis
70
-
 3 h, about 30% loss of activity Alcaligenes faecalis
80
-
 3 h, complete loss of activity Alcaligenes faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
-
 Alcaligenes faecalis

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6: about 40% of maximal activity, pH 6.5-8: about 80% of maximal activity Alcaligenes faecalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5
-
 30°C, 20 h, no loss of activity Alcaligenes faecalis
7
-
 30°C, 20 h, about 20% loss of activity Alcaligenes faecalis
8
-
 30°C, 20 h, about 30% loss of activity Alcaligenes faecalis
9
-
 30°C, 20 h, about 45% loss of activity Alcaligenes faecalis
10
-
 30°C, 20 h, about 75% loss of activity Alcaligenes faecalis