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Literature summary for 3.1.2.20 extracted from

  • Tilton, G.B.; Shockey, J.M.; Browse, J.
    Biochemical and molecular characterization of ACH2, an acyl-CoA thioesterase from arabidopsis thaliana (2004), J. Biol. Chem., 279, 7487-7494.
    View publication on PubMed
Search for more literature for 3.1.2.20

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis of isolated and commercial clone, overexpression of His-tagged isozyme ACH2 in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
acyl-CoA e.g. lauroyl-CoA, at 0.005 mM, can be overcome by addition of bovine serum albumin or alpha-casein Arabidopsis thaliana
CoASH feedback inhibition of recombinant enzyme at concentrations above 0.1 mM Arabidopsis thaliana
DTNB above 0.3 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Arabidopsis thaliana
0.0058
-
 palmitoleoyl-CoA pH 8.0 Arabidopsis thaliana
0.018
-
 dodecanoyl-CoA pH 8.0 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome isozyme ACH2 Arabidopsis thaliana 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
196000
-
 recombinant isozyme ACH2, analytical ultracentrifugation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + H2O Arabidopsis thaliana enzyme is not linked to fatty acid oxidation CoA + a carboxylate
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 isozyme ACH2
-
Arabidopsis thaliana
-
 Wassilewskija ecotype
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme ACH2 from Escherichia coli Arabidopsis thaliana

Reaction

Reaction Comment Organism Reaction ID
an acyl-CoA + H2O = CoA + a carboxylate catalytic triad D337-Q409-S359 Arabidopsis thaliana
a

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Arabidopsis thaliana
-
leaf young Arabidopsis thaliana
-
seedling
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21
-
 purified recombinant enzyme, substrate palmitoleoyl-CoA Arabidopsis thaliana

Storage Stability

Storage Stability Organism
4°C, purified recombinant HIs-tagged enzyme, pH 6.0, no loss of activity after 3 days Arabidopsis thaliana
4°C, purified recombinant HIs-tagged enzyme, pH 8.0, 90% remaining activity after 3 days Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + H2O prefers unsaturated acyl-CoA Arabidopsis thaliana CoA + a carboxylate
-
 ?
acyl-CoA + H2O long-chain fatty-acyl-CoA Arabidopsis thaliana CoA + a carboxylate
-
 ?
acyl-CoA + H2O enzyme is not linked to fatty acid oxidation Arabidopsis thaliana CoA + a carboxylate
-
 ?
dodecanoyl-CoA + H2O i.e. lauroyl-CoA Arabidopsis thaliana CoA + dodecanoate
-
 ?
palmitoleoyl-CoA + H2O best substrate Arabidopsis thaliana CoA + palmitoleoate
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Arabidopsis thaliana