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Literature summary for 3.1.2.20 extracted from

  • Maloberti, P.; Lozano, R.C.; Mele, P.G.; Cano, F.; Colonna, C.; Mendez, C.F.; Paz, C.; Podesta, E.J.
    Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells (2002), Eur. J. Biochem., 269, 5599-5607.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ACTH
-
Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
isozymes MTE-I, His-tagged, and CTE-I, both GST-fusion proteins Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NGDA isozymes CTE-I and MTE-I Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
Arachidonoyl-CoA isozyme MTE-I, pH 7.4, 37°C Homo sapiens
0.0041
-
Arachidonoyl-CoA isozyme CTE-I, pH 7.4, 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isozyme CTE I Homo sapiens 5829
-
mitochondrion isozyme MTE I Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
arachidonoyl-CoA + H2O Homo sapiens involved in cholesterol metabolism CoA + arachidonate
-
?
arachidonoyl-CoA + H2O Homo sapiens enzyme is responsible for the mobilization of stored arachidonic acid in cells CoA + arachidonate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isozymes CTE-I and MTE-I
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged isozymes CTE-I and MTE-I, this one also His-tagged, from Escherichia coli, to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland isozyme CTE-I Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
arachidonoyl-CoA + H2O
-
Homo sapiens CoA + arachidonate
-
?
arachidonoyl-CoA + H2O involved in cholesterol metabolism Homo sapiens CoA + arachidonate
-
?
arachidonoyl-CoA + H2O enzyme is responsible for the mobilization of stored arachidonic acid in cells Homo sapiens CoA + arachidonate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens