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Literature summary for 3.1.2.20 extracted from
- Purification and properties of acyl coenzyme A thioesterase II from Rhodopseudomonas sphaeroides (1986), Biochemistry, 25, 2480-2485.
General Stability
| General Stability | Organism |
|---|---|
| glycerol or ethylene glycol stabilize | Cereibacter sphaeroides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropylfluorophosphate | no inhibition | Cereibacter sphaeroides |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
octadecanoyl-CoA | pH 7.8, 22°C | Cereibacter sphaeroides | |
| 0.0005 | - |
hexadecanoyl-CoA | pH 7.8, 22°C | Cereibacter sphaeroides | |
| 0.0017 | - |
tetradecanoyl-CoA | pH 7.8, 22°C | Cereibacter sphaeroides | |
| 0.0018 | - |
dodecanoyl-CoA | pH 7.8, 22°C | Cereibacter sphaeroides | |
| 0.0027 | - |
decanoyl-CoA | pH 7.8, 22°C | Cereibacter sphaeroides | |
| 0.0055 | - |
hexanoyl-CoA | pH 7.8, 22°C | Cereibacter sphaeroides | |
| 0.007 | - |
octanoyl-CoA | pH 7.8, 22°C | Cereibacter sphaeroides | |
| 0.013 | - |
butyryl-CoA | pH 7.8, 22°C | Cereibacter sphaeroides | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Cereibacter sphaeroides | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
native PAGE | Cereibacter sphaeroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + H2O | Cereibacter sphaeroides | - |
CoA + a carboxylate | - |
? | |
| hexadecanoyl-CoA + H2O | Cereibacter sphaeroides | preferred substrate | CoA + hexadecanoate | - |
? | |
| hexadecanoyl-CoA + H2O | Cereibacter sphaeroides | i.e. palmitoyl-CoA | CoA + hexadecanoate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cereibacter sphaeroides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 5300fold | Cereibacter sphaeroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.6 | - |
purified enzyme, decanoyl-CoA as substrate | Cereibacter sphaeroides |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 20% v/v glycerol or ethylene glycol, t1/2: 1 year | Cereibacter sphaeroides |
| 4°C, in 50-70% ethylene glycol, t1/2: 60 days, upon removal of ethylene glycol: t1/2: 15 days | Cereibacter sphaeroides |
| room temperature, not more than 1 day | Cereibacter sphaeroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxydecanoyl-CoA + H2O | - |
Cereibacter sphaeroides | CoA + 3-hydroxydecanoate | - |
? | |
| acyl-CoA + H2O | - |
Cereibacter sphaeroides | CoA + a carboxylate | - |
? | |
| acyl-CoA + H2O | broad specificity for medium-chain to long-chain acyl-CoA | Cereibacter sphaeroides | CoA + a carboxylate | - |
? | |
| acyl-CoA + H2O | saturated and unsaturated acyl-CoA | Cereibacter sphaeroides | CoA + a carboxylate | - |
? | |
| decanoyl-CoA + H2O | - |
Cereibacter sphaeroides | CoA + decanoate | - |
? | |
| dodecanoyl-CoA + H2O | i.e. lauroyl-CoA | Cereibacter sphaeroides | CoA + dodecanoate | - |
? | |
| hexadecanoyl-CoA + H2O | best substrate | Cereibacter sphaeroides | CoA + hexadecanoate | - |
? | |
| hexadecanoyl-CoA + H2O | preferred substrate | Cereibacter sphaeroides | CoA + hexadecanoate | - |
? | |
| hexadecanoyl-CoA + H2O | i.e. palmitoyl-CoA | Cereibacter sphaeroides | CoA + hexadecanoate | - |
? | |
| hexanoyl-CoA + H2O | poor substrate | Cereibacter sphaeroides | CoA + hexanoate | - |
? | |
| n-butyryl-CoA + H2O | - |
Cereibacter sphaeroides | CoA + n-butanoate | - |
? | |
| octadecanoyl-CoA + H2O | preferred substrate | Cereibacter sphaeroides | CoA + octadecanoate | - |
? | |
| octadecanoyl-CoA + H2O | i.e. stearoyl-CoA | Cereibacter sphaeroides | CoA + octadecanoate | - |
? | |
| octanoyl-CoA + H2O | - |
Cereibacter sphaeroides | CoA + octanoate | - |
? | |
| tetradecanoyl-CoA + H2O | - |
Cereibacter sphaeroides | CoA + tetradecanoate | - |
? | |
| vaccenoyl-CoA + H2O | i.e. [trans]-11-octadecenoyl-CoA, preferred substrate of unsaturated acyl-CoA | Cereibacter sphaeroides | CoA + vaccenoate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Cereibacter sphaeroides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Cereibacter sphaeroides |
| 8.3 | 8.5 | - |
Cereibacter sphaeroides |
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