Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis, chromosome mapping 1p36.2, expression in Escherichia coli | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoic acid | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
hexadecanoyl-CoA | pH 7.4, 30°C | Homo sapiens | |
0.0076 | - |
dodecanoyl-CoA | pH 7.4, 30°C | Homo sapiens | |
0.0088 | - |
oleoyl-CoA | pH 7.4, 30°C | Homo sapiens | |
0.0092 | - |
Arachidonoyl-CoA | pH 7.4, 30°C | Homo sapiens | |
0.014 | - |
octanoyl-CoA | pH 7.4, 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
n * 43000, enzyme is a dimer or a trimer of MW 100 kDa, SDS-PAGE | Homo sapiens |
100000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00154 | human brain long-chain acyl-CoA hydrolase hBACH | - |
Purification (Comment) | Organism |
---|---|
from brain | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
198 | - |
purified native enzyme, substrate palmitoyl-CoA | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + H2O | acyl-CoA of chain length C8-C18 | Homo sapiens | CoA + a carboxylate | - |
? | |
acyl-CoA + H2O | long-chain fatty-acyl-CoA | Homo sapiens | CoA + a carboxylate | - |
? | |
arachidonoyl-CoA + H2O | - |
Homo sapiens | CoA + arachidonate | - |
? | |
dodecanoyl-CoA + H2O | - |
Homo sapiens | CoA + dodecanoate | - |
? | |
hexadecanoyl-CoA + H2O | best substrate | Homo sapiens | CoA + hexadecanoate | - |
? | |
hexadecanoyl-CoA + H2O | i.e. palmitoyl-CoA | Homo sapiens | CoA + hexadecanoate | - |
? | |
octanoyl-CoA + H2O | - |
Homo sapiens | CoA + octanoate | - |
? | |
oleoyl-CoA + H2O | - |
Homo sapiens | CoA + oleate | - |
? |
Subunits | Comment | Organism |
---|---|---|
oligomer | n * 43000, enzyme is a dimer or a trimer of MW 100 kDa, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |