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Literature summary for 3.1.2.20 extracted from
- Purification and properties of fatty acyl thioesterase I from Escherichia coli (1972), J. Biol. Chem., 247, 3123-3133.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-dinitrofluorobenzene | inhibits very slowly | Escherichia coli |  |
| iodoacetamide | - |
Escherichia coli | |
| methylene blue | photooxidation | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00013 | 0.006 | fatty-acyl-CoA thioester | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
4 * 30000, SDS-PAGE | Escherichia coli |
| 122000 | - |
gel filtration, thioesterase I | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-CoA + H2O | Escherichia coli | - |
CoA + palmitate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 8400fold | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 320 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + H2O | long-chain fatty-acyl-CoA | Escherichia coli | CoA + a carboxylate | - |
? | |
| decanoyl-CoA + H2O | - |
Escherichia coli | CoA + decanoate | - |
? | |
| hexadecanoyl-CoA + H2O | i.e. palmitoyl-CoA | Escherichia coli | CoA + hexadecanoate | - |
? | |
| hexanoyl-CoA + H2O | - |
Escherichia coli | CoA + hexanoate | - |
? | |
| octadecanoyl-CoA + H2O | i.e. stearoyl-CoA | Escherichia coli | CoA + octadecanoate | - |
? | |
| oleoyl pantetheine + H2O | - |
Escherichia coli | oleate + pantetheine | - |
? | |
| oleoyl-CoA + H2O | - |
Escherichia coli | CoA + oleate | - |
? | |
| oleyl-[acyl-carrier protein] + H2O | - |
Escherichia coli | oleate + acyl-carrier protein | - |
? | |
| palmitoleoyl-CoA + H2O | - |
Escherichia coli | CoA + palmitoleoate | - |
? | |
| palmitoyl-CoA + H2O | - |
Escherichia coli | CoA + palmitate | - |
? | |
| tetradecanoyl-CoA + H2O | i.e. myristoyl-CoA | Escherichia coli | CoA + tetradecanoate | - |
? | |
| vaccenoyl-CoA + H2O | cis-isomer | Escherichia coli | CoA + vaccenoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 30000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.8 | - |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
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