Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.2.2 extracted from

  • Lo, Y.C.; Lee, Y.L.; Shaw, J.F.; Liaw, Y.C.
    Crystallization and preliminary X-ray crystallographic analysis of thioesterase I from Escherichia coli (2000), Acta Crystallogr. Sect. D, 56, 756-757.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme, 76.8 mg/ml, hanging-drop vapour-diffusion method, protein solution: 10 mM sodium phosphate, pH 7.0, plus equal volme of reservoir solution: 0.2 M ammonium sulfate, 27% w/w PEG-MME 5K, 0.1 M 2-[N-morpholino]ethanesulfonic acid, pH 6.5, room temperature, crystals appeared after 7-21 d, X-ray diffraction structure determination and analysis at 2.4 A resolution Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
palmitoyl-CoA + H2O = CoA + palmitate specific catalyzation of the deacylation of long-chain fatty acyl-CoA thioesters, enzyme possesses a Ser-His-Asp catalytic triad Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
palmitoyl-CoA + H2O
-
Escherichia coli CoA + palmitate
-
?

Synonyms

Synonyms Comment Organism
thioesterase I
-
Escherichia coli