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Literature summary for 3.1.2.2 extracted from
- Two families of acyl-CoA thioesterases in Arabidopsis (2000), Biochem. Soc. Trans., 28, 946-947.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | enzyme is involved in fatty acid biosynthesis and may be a good target for improvement of special oil production in transgenic plants | Arabidopsis thaliana |
| biotechnology | enzyme can be used for bioengineering of transgenic plants to produce oils with desired properties | Arabidopsis thaliana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional overexpression of AtACH2 in Escherichia coli, overexpression of AtACH5 fused to the green fluorescent protein in Escherichia coli | Arabidopsis thaliana |
| overexpression of isozymes AtACH5and AtACH2 in Escherichia coli , AtACH2 fused to maltose-binding protein | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | a AtACH5 gene knock-out mutant shows no altered phenotype | Arabidopsis thaliana |
| additional information | AtACH5 knockout mutants do not have an altered phenotype | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | isozymes AtACH4 and AtACH5 | Arabidopsis thaliana | 5783 | - |
| endoplasmic reticulum | isozyme family built of ACH4 and ACH5 | Arabidopsis thaliana | 5783 | - |
| peroxisome | isozymes AtACH1 and AtACH2 | Arabidopsis thaliana | 5777 | - |
| peroxisome | isozyme family built of ACH1 and ACH2 | Arabidopsis thaliana | 5777 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + H2O | Arabidopsis thaliana | - |
CoA + a carboxylate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
2 families of acyl-CoA thioesterases due to their localization: 1. isozymes ACH1 and ACH2, 2. isozymes ACH4 and ACH5 | - |
| Arabidopsis thaliana | - |
2 families of acyl-CoA thioesterases: 1. peroxisomal family comprising ACH1 and ACH2, 2. endoplasmic family comprising ACH4 and ACH5 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial purification of recombinant ACH2 overexpressed in Escherichia coli | Arabidopsis thaliana |
| partially, recombinant isozyme AtACH2 fused to maltose-binding protein from Escherichia coli | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + a carboxylate | - |
? | |
| hexadecanoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + hexadecanoate | - |
? | |
| oleoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + oleate | - |
? | |
| palmitoyl-CoA + H2O | - |
Arabidopsis thaliana | CoA + palmitate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACH | - |
Arabidopsis thaliana |
| acyl-CoA thioesterase | - |
Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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