Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | a small amount activates the hydrolase | Rattus norvegicus |
General Stability | Organism |
---|---|
30% ethylene glycol, 50% loss of activity after 5 days | Rattus norvegicus |
purified enzyme needs 30% v/v ethylene glycol for stabilization | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-acyl-sn-glycerol-3-phosphocholine | competitive inhibition at low concentration of palmitoyl-CoA | Rattus norvegicus | |
bovine serum albumin | a high amount inhibits the hydrolase | Rattus norvegicus | |
Ca2+ | 50% inhibition at 1.6 mM | Rattus norvegicus | |
diethyldicarbonate | inactivation | Rattus norvegicus | |
L-palmitoyl carnitine | competitive to acyl-CoA | Rattus norvegicus | |
lysophosphatidylcholine | - |
Rattus norvegicus | |
lysophosphatidylinositol | - |
Rattus norvegicus | |
methyl methanethiosulfonate | slight inhibition | Rattus norvegicus | |
Mg2+ | 50% inhibition at 1.6 mM | Rattus norvegicus | |
additional information | no inhibition by NEM, iodoacetamide, L-carnitine | Rattus norvegicus | |
octylglucoside | 50% inhibition at 0.3% | Rattus norvegicus | |
p-hydroxymercuribenzoate | - |
Rattus norvegicus | |
PMSF | no inhibition | Rattus norvegicus | |
SDS | 50% inhibition at 0.035% | Rattus norvegicus | |
sodium deoxycholate | 50% inhibition at 0.04% | Rattus norvegicus | |
Triton X-100 | 50% inhibition at 0.01% | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
dodecanoyl-CoA | pH 7.5, 37°C | Rattus norvegicus | |
0.0046 | - |
Arachidonoyl-CoA | pH 7.5, 37°C | Rattus norvegicus | |
0.0058 | - |
hexadecanoyl-CoA | pH 7.5, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40900 | - |
4 * 40900, SDS-PAGE | Rattus norvegicus |
40900 | - |
x * 40900, SDS-PAGE | Rattus norvegicus |
148000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hexadecanoyl-CoA + H2O | Rattus norvegicus | i.e. palmitoyl-CoA | CoA + hexadecanoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
3500fold to homogeneity from brain cytosol | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1478 | - |
purified enzyme, substrate palmitoyl-CoA | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C in the presence of 20% glycerol over 1 month | Rattus norvegicus |
-70°C, partially purified enzyme, at least 1 month without loss of activity | Rattus norvegicus |
-70°C, pure enzyme, 0.1 M potassium phosphate, pH 7.5, 30% v/v ethylene glycol, loss of 50% activity within 5 days | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + H2O | 8% activity compared to palmitoyl-CoA | Rattus norvegicus | CoA + acetoacetate | - |
? | |
acetyl-CoA + H2O | no activity | Rattus norvegicus | CoA + acetate | - |
? | |
acyl-CoA + H2O | broad specificity for medium-chain to long-chain acyl-CoA | Rattus norvegicus | CoA + a carboxylate | - |
? | |
acyl-CoA + H2O | saturated and unsaturated acyl-CoA | Rattus norvegicus | CoA + a carboxylate | - |
? | |
acyl-CoA + H2O | specific for acyl-CoA, S-palmitoyl-glutathione and S-palmitoyl-cysteine are no substrates | Rattus norvegicus | CoA + a carboxylate | - |
? | |
arachidonoyl-CoA + H2O | i.e. all-cis-5,8,11,14-eicosatetranoate | Rattus norvegicus | CoA + arachidonate | - |
? | |
dodecanoyl-CoA + H2O | best substrate | Rattus norvegicus | CoA + dodecanoate | - |
? | |
dodecanoyl-CoA + H2O | i.e. lauroyl-CoA | Rattus norvegicus | CoA + dodecanoate | - |
? | |
hexadecanoyl-CoA + H2O | i.e. palmitoyl-CoA | Rattus norvegicus | CoA + hexadecanoate | - |
? | |
additional information | no activity with malonyl-CoA, ligneceroyl-CoA, and nervonoyl-CoA | Rattus norvegicus | ? | - |
? | |
n-butyryl-CoA + H2O | no activity | Rattus norvegicus | CoA + n-butanoate | - |
? | |
tetradecanoyl-CoA + H2O | - |
Rattus norvegicus | CoA + tetradecanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40900, SDS-PAGE | Rattus norvegicus |
tetramer | 4 * 40900, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |