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Literature summary for 3.1.2.2 extracted from

  • Broustas, C.G.; Hajra, A.K.
    Purification, properties, and specificity of rat brain cytosolic fatty acyl coenzyme A hydrolase (1995), J. Neurochem., 64, 2345-2353.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin a small amount activates the hydrolase Rattus norvegicus

General Stability

General Stability Organism
30% ethylene glycol, 50% loss of activity after 5 days Rattus norvegicus
purified enzyme needs 30% v/v ethylene glycol for stabilization Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1-acyl-sn-glycerol-3-phosphocholine competitive inhibition at low concentration of palmitoyl-CoA Rattus norvegicus
bovine serum albumin a high amount inhibits the hydrolase Rattus norvegicus
Ca2+ 50% inhibition at 1.6 mM Rattus norvegicus
diethyldicarbonate inactivation Rattus norvegicus
L-palmitoyl carnitine competitive to acyl-CoA Rattus norvegicus
lysophosphatidylcholine
-
Rattus norvegicus
lysophosphatidylinositol
-
Rattus norvegicus
methyl methanethiosulfonate slight inhibition Rattus norvegicus
Mg2+ 50% inhibition at 1.6 mM Rattus norvegicus
additional information no inhibition by NEM, iodoacetamide, L-carnitine Rattus norvegicus
octylglucoside 50% inhibition at 0.3% Rattus norvegicus
p-hydroxymercuribenzoate
-
Rattus norvegicus
PMSF no inhibition Rattus norvegicus
SDS 50% inhibition at 0.035% Rattus norvegicus
sodium deoxycholate 50% inhibition at 0.04% Rattus norvegicus
Triton X-100 50% inhibition at 0.01% Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0038
-
dodecanoyl-CoA pH 7.5, 37°C Rattus norvegicus
0.0046
-
Arachidonoyl-CoA pH 7.5, 37°C Rattus norvegicus
0.0058
-
hexadecanoyl-CoA pH 7.5, 37°C Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Rattus norvegicus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40900
-
4 * 40900, SDS-PAGE Rattus norvegicus
40900
-
x * 40900, SDS-PAGE Rattus norvegicus
148000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hexadecanoyl-CoA + H2O Rattus norvegicus i.e. palmitoyl-CoA CoA + hexadecanoate
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3500fold to homogeneity from brain cytosol Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1478
-
purified enzyme, substrate palmitoyl-CoA Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C in the presence of 20% glycerol over 1 month Rattus norvegicus
-70°C, partially purified enzyme, at least 1 month without loss of activity Rattus norvegicus
-70°C, pure enzyme, 0.1 M potassium phosphate, pH 7.5, 30% v/v ethylene glycol, loss of 50% activity within 5 days Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + H2O 8% activity compared to palmitoyl-CoA Rattus norvegicus CoA + acetoacetate
-
?
acetyl-CoA + H2O no activity Rattus norvegicus CoA + acetate
-
?
acyl-CoA + H2O broad specificity for medium-chain to long-chain acyl-CoA Rattus norvegicus CoA + a carboxylate
-
?
acyl-CoA + H2O saturated and unsaturated acyl-CoA Rattus norvegicus CoA + a carboxylate
-
?
acyl-CoA + H2O specific for acyl-CoA, S-palmitoyl-glutathione and S-palmitoyl-cysteine are no substrates Rattus norvegicus CoA + a carboxylate
-
?
arachidonoyl-CoA + H2O i.e. all-cis-5,8,11,14-eicosatetranoate Rattus norvegicus CoA + arachidonate
-
?
dodecanoyl-CoA + H2O best substrate Rattus norvegicus CoA + dodecanoate
-
?
dodecanoyl-CoA + H2O i.e. lauroyl-CoA Rattus norvegicus CoA + dodecanoate
-
?
hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Rattus norvegicus CoA + hexadecanoate
-
?
additional information no activity with malonyl-CoA, ligneceroyl-CoA, and nervonoyl-CoA Rattus norvegicus ?
-
?
n-butyryl-CoA + H2O no activity Rattus norvegicus CoA + n-butanoate
-
?
tetradecanoyl-CoA + H2O
-
Rattus norvegicus CoA + tetradecanoate
-
?

Subunits

Subunits Comment Organism
? x * 40900, SDS-PAGE Rattus norvegicus
tetramer 4 * 40900, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Rattus norvegicus