Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.2.2 extracted from

  • Boyce, S.G.; Luecking, D.R.
    Purification and characterization of long-chain acyl coenzyme A thioesterase from Rhodopseudomonas sphaeroides (1984), Biochemistry, 23, 141-147.
No PubMed abstract available
Search for more literature for 3.1.2.2

General Stability

General Stability Organism
glycerol and palmitoyl-CoA stabilize Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
diisopropylfluorophosphate 0.01 mM inhibits Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000158
-
 hexadecanoyl-CoA
-
 Cereibacter sphaeroides
0.00084
-
 vaccenoyl-CoA
-
 Cereibacter sphaeroides
0.0014
-
 linoleoyl-CoA
-
 Cereibacter sphaeroides
0.00162
-
 oleoyl-CoA
-
 Cereibacter sphaeroides
0.00186
-
 hexadecanoyl-CoA
-
 Cereibacter sphaeroides
0.0046
-
 myristoleoyl-CoA
-
 Cereibacter sphaeroides
0.00786
-
 linolenoyl-CoA
-
 Cereibacter sphaeroides
0.00867
-
 tetradecanoyl-CoA
-
 Cereibacter sphaeroides
0.04468
-
 dodecanoyl-CoA
-
 Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12500
-
 2 * 12500, SDS-PAGE Cereibacter sphaeroides
22400
-
 gel filtration, thioesterase I Cereibacter sphaeroides
24000
-
 gel filtration, sedimentation velocity Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
palmitoyl-CoA + H2O Cereibacter sphaeroides
-
 CoA + palmitate
-
 ?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
10000fold Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
67.5
-
-
 Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-20°C over 1 month Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dodecanoyl-CoA + H2O i.e. lauroyl-CoA Cereibacter sphaeroides CoA + dodecanoate
-
 ?
hexadecanoyl-CoA + H2O i.e. palmitoyl-CoA Cereibacter sphaeroides CoA + hexadecanoate
-
 ?
linolenoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + linolenoate
-
 ?
linoleoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + linoleate
-
 ?
myristoleoyl-CoA + H2O
-
 Cereibacter sphaeroides myristoleoate + CoA
-
 ?
myristoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + myristate
-
 ?
n-butyryl-CoA + H2O
-
 Cereibacter sphaeroides CoA + n-butanoate
-
 ?
octadecanoyl-CoA + H2O i.e. stearoyl-CoA Cereibacter sphaeroides CoA + octadecanoate
-
 ?
oleoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + oleate
-
 ?
palmitoleoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + palmitoleoate
-
 ?
palmitoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + palmitate
-
 ?
tetradecanoyl-CoA + H2O i.e. myristoyl-CoA Cereibacter sphaeroides CoA + tetradecanoate
-
 ?
vaccenoyl-CoA + H2O
-
 Cereibacter sphaeroides CoA + vaccenoate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 12500, SDS-PAGE Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Cereibacter sphaeroides