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Literature summary for 3.1.2.17 extracted from
- D-Methylmalonyl-CoA hydrolase (1988), Methods Enzymol., 166, 393-400.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
(S)-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
- |
Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.41 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, purified enzyme stable for at least 1 month | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-methylmalonyl-CoA + H2O | not active on the L-isomer | Rattus norvegicus | methylmalonate + CoA | - |
? | |
| malonyl-CoA + H2O | 16% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | malonate + CoA | - |
? | |
| additional information | acetyl-CoA 1% of the activity with (S)-methylmalonyl-CoA , succinyl-CoA and palmitoyl-CoA hydrolyzed at less than 1% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | ? | - |
? | |
| propionyl-CoA + H2O | 3% of the activity with (S)-methylmalonyl-CoA | Rattus norvegicus | propionate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE, in absence and presence of 1% 2-mercaptoethanol | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-methylmalonyl-CoA hydrolase | - |
Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 23.3 | - |
(S)-methylmalonyl-CoA | pH 6.0, 30°C | Rattus norvegicus | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | - |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactors identified | Rattus norvegicus |
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Release 2023.1 (January 2023)
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